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文献和实验Assessment of Drug Transporter Function Using Fluorescent Cell Imaging
Introduction Basic Protocol 1: Measurement of Transporter Function in Cells Overexpressing an ABC Transporter Using an Automated Fluorescent Cell Counter Alternate Protocol 1: Measurement
tubes, remove air bubbles, seal with rubber stoppers, and crimp properly. 11a. Centrifuge at 60,000 rpm to 16-20 hours at 15-20degC in the Sorvall OTD-75B ultracentrifuge (DuPont) using the T-865 rotor. 12a. Visualize the ethidium bromide
Nitrogen Freezer Materials Cryovials, 1.8 ml (Nunc or equivalent) Cryovial rack (Nunc or equivalent) Sterile Centrifuge tubes, 50 ml (VWR # 21008-146 or equivalent) Fetal Bovine Serum, heat inactivated (BioWhittaker # 14-503F
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