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- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pAM34
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: pAM34
其他名称:
质粒大小（bp）: 6000
抗性: Ampicillin
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号: M69063
其他属性: Permits positive selection because deletion of the aadA fragment is not viable. pAM35 (ATCC 77186) differs from pAM34 (ATCC 77185) by deletion of the DraII fragment in pAM34 carrying lacIq. The order of the major features in this plasmid is: lacZpo – ori – rom – transcription terminator- BamHI – MCS – XmaIII – aadA (spcR) – MCS – BamHI – transcription terminator – bla – lacIq. [1] Restriction digests of the clone give the following sizes (kb): EcoRI–6.0; BamHI–4.3,1.8; HindIII–6.0; PstI–6.0; KpnI–3.4, 2.7. (ATCC staff) Medium is -1 1227 + IPTG (1 mM). Hosts: E.coli JS219, E.coli.
质粒图谱:
质粒序列:
| 1 | ctagagtcgc | aacgcaatta | atgtgagtta | gctcactcat | taggcacccc | 50 |
| 51 | aggctttaca | ctttatgctt | ccggctcgta | tgttgtgtgg | aattgtgagc | 100 |
| 101 | ggataacaat | ttcacacagg | aaacaggggt | accctgcttg | caaacaaaaa | 150 |
| 151 | aaccaccgct | accagcggtg | gtttgtttgc | cggatcaaga | gctaccaact | 200 |
| 201 | ctttttccga | aggtaactgg | cttcagcaga | gcgcagatac | caaatactgt | 250 |
| 251 | ccttctagtg | tagccgtagt | taggccacca | cttcaagaac | tctgtagcac | 300 |
| 301 | cgcctacata | cctcgctctg | ctaatcctgt | taccagtggc | tgctgccagt | 350 |
| 351 | ggcgataagt | cgtgtcttac | cgggttggac | tcaagacgat | agttaccgga | 400 |
| 401 | taaggcgcag | cggtcgggct | gaacgggggg | ttcgtgcaca | cagcccagct | 450 |
| 451 | tggagcgaac | gacctacacc | gaactgagat | acctacagcg | tgagctatga | 500 |
| 501 | gaaagcgcca | cgcttcccga | agggagaaag | gcggacaggt | atccggtaag | 550 |
| 551 | cggcagggtc | ggaacaggag | agcgcacgag | ggagcttcca | gggggaaacg | 600 |
| 601 | cctggtatct | ttatagtcct | gtcgggtttc | gccacctctg | acttgagcgt | 650 |
| 651 | cgatttttgt | gatgctcgtc | aggggggcgg | agcctatgga | aaaacgccag | 700 |
| 701 | caacgcggcc | tttttacggt | tcctggcctt | ttgctggcct | tttgctcaca | 750 |
| 751 | tgttctttcc | tgcgttatcc | cctgattctg | tggataaccg | tattaccgcc | 800 |
| 801 | tttgagtgag | ctgataccgc | tcgccgcagc | cgaacgaccg | agcgcagcga | 850 |
| 851 | gtcagtgagc | gaggaagcgg | aagagcgcct | gatgcggtat | tttctcctta | 900 |
| 901 | cgcatctgtg | cggtatttca | caccgcatat | ggtgcactct | cagtacaatc | 950 |
| 951 | tgctctgatg | ccgcatagtt | aagccagtat | acactccgct | atcgctacgt | 1000 |
| 1001 | gactgggtca | tggctgcgcc | ccgacacccg | ccaacacccg | ctgacgcgcc | 1050 |
| 1051 | ctgacgggct | tgtctgctcc | cggcatccgc | ttacagacaa | gctgtgaccg | 1100 |
| 1101 | tctccgggag | ctgcatgtgt | cagaggtttt | caccgtcatc | accgaaacgc | 1150 |
| 1151 | gcgaggcagc | tgcggtaaag | ctcatcagcg | tggtcgtgaa | gcgattcaca | 1200 |
| 1201 | gatgtctgcc | tgttcatccg | cgtccagctc | gttgagtttc | tccagaagcg | 1250 |
| 1251 | ttaatgtctg | gcttctgata | aagcgggcca | tgttaagggc | ggttttttcc | 1300 |
| 1301 | tgtttggtca | ctgatgcctc | cgtgtaaggg | ggaattctgt | tcatgggggt | 1350 |
| 1351 | aatgataccg | atgaaacgag | agaggatgct | cacgatacgg | gttactgatg | 1400 |
| 1401 | atgaacatgc | ccggttactg | gaacgttgtg | agggtaaaca | actggcggta | 1450 |
| 1451 | tggatgcggc | gggaccagag | aaaaatcact | cagggtcaat | gccagcgctt | 1500 |
| 1501 | cgttaataca | gatgtaggtg | ttccacaggg | tagccagcag | catcctgcga | 1550 |
| 1551 | tgcagatccg | gaacataatg | gtgcagggcg | ctgacttccg | cgtttccaga | 1600 |
| 1601 | ctttacgaaa | cacggaaacc | gaagaccatt | catgttgttg | ctcaggtcgc | 1650 |
| 1651 | agacgttttg | cagcagcagt | cgcttcacgt | tcgctcgcgt | atcggtgatt | 1700 |
| 1701 | cattctgcta | accagtaagg | caaccccgcc | agcctagatc | cttttagctt | 1750 |
| 1751 | tatgcttgta | aaccgttttg | tgaaaaaatt | tttaaaataa | aaaaggggac | 1800 |
| 1801 | ctctagggtc | cccaattaat | tagtaatata | atctattaaa | ggtcattcaa | 1850 |
| 1851 | aaggtcatcc | accggatcct | ctagagtcga | cctgcaggca | tgcccgttcc | 1900 |
| 1901 | atacagaagc | tgggcgaaca | aacgatgctc | gccttccaga | aaaccgagga | 1950 |
| 1951 | tgcgaaccac | ttcatccggg | gtcagcacca | ccggcaagcg | ccgcgacggc | 2000 |
| 2001 | cgaggtcttc | cgatctcctg | aagccagggc | agatccgtgc | acagcacctt | 2050 |
| 2051 | gccgtagaag | aacagcaagg | ccgccaatgc | ctgacgatgc | gtggagaccg | 2100 |
| 2101 | aaaccttgcg | ctcgttcgcc | agccaggaca | gaaatgcctc | gacttcgctg | 2150 |
| 2151 | ctgcccaagg | ttgccgggtg | acgcacaccg | tggaaacgga | tgaaggcacg | 2200 |
| 2201 | aacccagtgg | acataagcct | gttcggttcg | taagctgtaa | tgcaagtagc | 2250 |
| 2251 | gtatgcgctc | acgcaactgg | tccagaacct | tgaccgaacg | cagcggtggt | 2300 |
| 2301 | aacggcgcag | tggcggtttt | catggcttgt | tatgactgtt | tttttggggt | 2350 |
| 2351 | acagtctatg | cctcgggcat | ccaagcagca | agcgcgttac | gccgtgggtc | 2400 |
| 2401 | gatgtttgat | gttatggagc | agcaacgatg | ttacgcagca | gggcagtcgc | 2450 |
| 2451 | cctaaaacaa | agttaaacat | catgagggaa | gcggtgatcg | ccgaagtatc | 2500 |
| 2501 | gactcaacta | tcagaggtag | ttggcgtcat | cgagcgccat | ctcgaaccga | 2550 |
| 2551 | cgttgctggc | cgtacatttg | tacggctccg | cagtggatgg | cggcctgaag | 2600 |
| 2601 | ccacacagtg | atattgattt | gctggttacg | gtgaccgtaa | ggcttgatga | 2650 |
| 2651 | aacaacgcgg | cgagctttga | tcaacgacct | tttggaaact | tcggcttccc | 2700 |
| 2701 | ctggagagag | cgagattctc | cgcgctgtag | aagtcaccat | tgttgtgcac | 2750 |
| 2751 | gacgacatca | ttccgtggcg | ttatccagct | aagcgcgaac | tgcaatttgg | 2800 |
| 2801 | agaatggcag | cgcaatgaca | ttcttgcagg | tatcttcgag | ccagccacga | 2850 |
| 2851 | tcgacattga | tctggctatc | ttgctgacaa | aagcaagaga | acatagcgtt | 2900 |
| 2901 | gccttggtag | gtccagcggc | ggaggaactc | tttgatccgg | ttcctgaaca | 2950 |
| 2951 | ggatctattt | gaggcgctaa | atgaaacctt | aacgctatgg | aactcgccgc | 3000 |
| 3001 | ccgactgggc | tggcgatgag | cgaaatgtag | tgcttacgtt | gtcccgcatt | 3050 |
| 3051 | tggtacagcg | cagtaaccgg | caaaatcgcg | ccgaaggatg | tcgctgccga | 3100 |
| 3101 | ctgggcaatg | gagcgcctgc | cggcccagta | tcagcccgtc | atacttgaag | 3150 |
| 3151 | ctagacaggc | ttatcttgga | caagaagaag | atcgcttggc | ctcgcgcgca | 3200 |
| 3201 | gatcagttgg | aagaatttgt | ccactacgtg | aaaggcgaga | tcaccaaggt | 3250 |
| 3251 | agtcggcaaa | taatgtctaa | caattcgttc | aagccgacgc | cgcttcgcgg | 3300 |
| 3301 | cgcggcttaa | ctcaagcgtt | agatgcacta | agcacataat | tgctcacagc | 3350 |
| 3351 | caaactatca | ggtcaagtct | gcttttatta | tttttaagcg | tgcataataa | 3400 |
| 3401 | gccctacaca | aattgggaga | tatatcatga | aaggctggct | ttttcttgtt | 3450 |
| 3451 | atcgcaatag | ttggcgaagt | aatcgcaaca | tccgcattaa | aatctagcga | 3500 |
| 3501 | gggctttact | aagcttatcg | aattcgagct | cggtaccccg | gggatccggt | 3550 |
| 3551 | ggatgacctt | ttgaatgacc | tttaatagat | tatattacta | attaattggg | 3600 |
| 3601 | gaccctagag | gtcccctttt | ttattttaaa | aattttttca | caaaacggtt | 3650 |
| 3651 | tacaagcata | aagctaaatc | aatctaaagt | atatatgagt | aaacttggtc | 3700 |
| 3701 | tgacagttac | caatgcttaa | tcagtgaggc | acctatctca | gcgatctgtc | 3750 |
| 3751 | tatttcgttc | atccatagtt | gcctgactcc | ccgtcgtgta | gataactacg | 3800 |
| 3801 | atacgggagg | gcttaccatc | tggccccagt | gctgcaatga | taccgcgaga | 3850 |
| 3851 | cccacgctca | ccggctccag | atttatcagc | aataaaccag | ccagccggaa | 3900 |
| 3901 | gggccgagcg | cagaagtggt | cctgcaactt | tatccgcctc | catccagtct | 3950 |
| 3951 | attaattgtt | gccgggaagc | tagagtaagt | agttcgccag | ttaatagttt | 4000 |
| 4001 | gcgcaacgtt | gttgccattg | ctacaggcat | cgtggtgtca | cgctcgtcgt | 4050 |
| 4051 | ttggtatggc | ttcattcagc | tccggttccc | aacgatcaag | gcgagttaca | 4100 |
| 4101 | tgatccccca | tgttgtgcaa | aaaagcggtt | agctccttcg | gtcctccgat | 4150 |
| 4151 | cgttgtcaga | agtaagttgg | ccgcagtgtt | atcactcatg | gttatggcag | 4200 |
| 4201 | cactgcataa | ttctcttact | gtcatgccat | ccgtaagatg | cttttctgtg | 4250 |
| 4251 | actggtgagt | actcaaccaa | gtcattctga | gaatagtgta | tgcggcgacc | 4300 |
| 4301 | gagttgctct | tgcccggcgt | caacacggga | taataccgcg | ccacatagca | 4350 |
| 4351 | gaactttaaa | agtgctcatc | attggaaaac | gttcttcggg | gcgaaaactc | 4400 |
| 4401 | tcaaggatct | taccgctgtt | gagatccagt | tcgatgtaac | ccactcgtgc | 4450 |
| 4451 | acccaactga | tcttcagcat | cttttacttt | caccagcgtt | tctgggtgag | 4500 |
| 4501 | caaaaacagg | aaggcaaaat | gccgcaaaaa | agggaataag | ggcgacacgg | 4550 |
| 4551 | aaatgttgaa | tactcatact | cttccttttt | caatattatt | gaagcattta | 4600 |
| 4601 | tcagggttat | tgtctcatga | gcggatacat | atttgaatgt | atttagaaaa | 4650 |
| 4651 | ataaacaaat | aggggttccg | cgcacatttc | cccgaaaagt | gccacctgac | 4700 |
| 4701 | gtctaagaaa | ccattattat | catgacatta | acctataaaa | ataggcgtat | 4750 |
| 4751 | cacgaggccc | tttcgtcttc | aagggtcgct | cactgcccgc | tttccagtcg | 4800 |
| 4801 | ggaaacctgt | cgtgccagct | gcattaatga | atcggccaac | gcgcggggag | 4850 |
| 4851 | aggcggtttg | cgtattgggc | gccagggtgg | tttttctttt | caccagtgag | 4900 |
| 4901 | acgggcaaca | gctgattgcc | cttcaccgcc | tggccctgag | agagttgcag | 4950 |
| 4951 | caagcggtcc | acgctggttt | gccccagcag | gcgaaaatcc | tgtttgatgg | 5000 |
| 5001 | tggttgacgg | cgggatataa | catgagctgt | cttcggtatc | gtcgtatccc | 5050 |
| 5051 | actaccgaga | tatccgcacc | aacgcgcagc | ccggactcgg | taatggcgcg | 5100 |
| 5101 | cattgcgccc | agcgccatct | gatcgttggc | aaccagcatc | gcagtgggaa | 5150 |
| 5151 | cgatgccctc | attcagcatt | tgcatggttt | gttgaaaacc | ggacatggca | 5200 |
| 5201 | ctccagtcgc | cttcccgttc | cgctatcggc | tgaatttgat | tgcgagtgag | 5250 |
| 5251 | atatttatgc | cagccagcca | gacgcagacg | cgccgagaca | gaacttaatg | 5300 |
| 5301 | ggcccgctaa | cagcgcgatt | tgctggtgac | ccaatgcgac | cagatgctcc | 5350 |
| 5351 | acgcccagtc | gcgtaccgtc | ttcatgggag | aaaataatac | tgttgatggg | 5400 |
| 5401 | tgtctggtca | gagacatcaa | gaaataacgc | cggaacatta | gtgcaggcag | 5450 |
| 5451 | cttccacagc | aatggcatcc | tggtcatcca | gcggatagtt | aatgatcagc | 5500 |
| 5501 | ccactgacgc | gttgcgcgag | aagattgtgc | accgccgctt | tacaggcttc | 5550 |
| 5551 | gacgccgctt | cgttctacca | tcgacaccac | cacgctggca | cccagttgat | 5600 |
| 5601 | cggcgcgaga | tttaatcgcc | gcgacaattt | gcgacggcgc | gtgcagggcc | 5650 |
| 5651 | agactggagg | tggcaacgcc | aatcagcaac | gactgtttgc | ccgccagttg | 5700 |
| 5701 | ttgtgccacg | cggttgggaa | tgtaattcag | ctccgccatc | gccgcttcca | 5750 |
| 5751 | ctttttcccg | cgttttcgca | gaaacgtggc | tggcctggtt | caccacgcgg | 5800 |
| 5801 | gaaacggtct | gataagagac | accggcatac | tctgcgacat | cgtataacgt | 5850 |
| 5851 | tactggtttc | acattcacca | ccctgaattg | actctcttcc | gggcgctatc | 5900 |
| 5901 | atgccatacc | gcgaaaggtt | ttgcaccatt | cgatggtgtc | ggcagcgttg | 5950 |
| 5951 | ggtcctggcc | acgggtgcgc | atgatcgtgc | tcctgtcgtt | gaggacccgg | 6000 |
start=
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