万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
lambda Pop6(lambdaPop6)
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: lambda Pop6
其他名称:
质粒大小(bp):
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号: X03453, M22642, M19164, M19165, M19166, M19167, M19168
其他属性: Hosts: E.coli 293-EBNA. Related vectors: EBV, lambda.
质粒图谱:
质粒序列:
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26, 1993 Background : There are many published methods for mini-preps of DNA from lambda phage cloning vectors. This one has worked
1. Grow a fresh overnight culture of lambda host cells (we use K803 for EMBL3) in LB or T-broth fortified with 10mM Mg++ . 2. Mix a small amount (1 to 20μl, aim for an M.O.I. of +0.01) of high titer phage lysate (e.g. from a plate lysate
in which the region including lacZ and multiple cloning sites was replaced by lambda cos sequence). In vivo homologous recombination between two vectors via cos sites resulted in pFOS1. The vector is extremely unstable in most of E. coli strains
技术资料暂无技术资料 索取技术资料




