lambda NMT

Vector for efficient transduction of mammalian cells with a cDNA library constructed with the pcD (Okayama and Berg) expression vector. The order of the major features in this bacteriophage is: lambda762/deltab – HindIII – 0.5 kb SV40 fragment, map units 0.86-0.95 – reddelta – SalI – SV40 ori – SV40 early promoter – SV40 late 19S and 16S splice junctions – neo (Tn5) – SV40 late poly(A) – pMB1 ori – ampR – lambda762/KH54/lambda ori/nin5/QSR. Cloning capacity:12 kb, or 9 kb of cDNA insert and 3 kb of pcD vector. Medium is 1592 SM buffer. Hosts: bacteria-free lysate, vertebrate cells, E.coli, E.coli JC8679 [ATCC 47001 for library amplification], E.coli C600 [ATCC 23724 for vector amplification].