- ￥1700 - 4000
- GeneTex
- 美国
- GTX113466
- 2025年07月11日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX113466
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HPRT
- 抗体英文名：
HPRT antibody
- 抗体名：
HPRT 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HPRT antibody detects HPRT protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunofluorescence analysis of PFA-fixed HeLa, using HPRT(GTX113466) antibody at 1:200 dilution.
HPRT antibody detects HPRT protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HPRT antibody detects HPRT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HPRT antibody detects HPRT protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
HPRT antibody detects HPRT1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
12% SDS-PAGE
HPRT antibody (GTX113466) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HPRT antibody detects HPRT protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody (GTX113466) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
HPRT stained by HPRT antibody (GTX113466) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Teraishi M et al., Sci Rep 2017 (PMID:28422173)
Molecular Analysis of Mutations in the Human HPRT Gene
The HPRT assay uses incorporation of toxic nucleotide analogs to select for cells lacking the purine scavenger enzyme hypoxanthine-guanine phosporibosyltransferase. A major advantage of this assay is the ability to isolate mutant cells
Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
is required for the formation of functional T-cell receptor genes in T cells and antibody genes in B cells. This is an inherently dangerous process, however, because double-strand breaks are introduced into the chromosomes. Molecular evidence indicates
Somatic Mutations and Aging: Methods for Molecular Analysis of HPRT Mutations
susceptibility factors seem to contribute to the complexity of mutational spectra. The X-linked HPRT (hypoxanthine phosphoribosyl transferase ) gene in human T lymphocytes has been considered as a suitable target for studying somatic in vivo mutations
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