- ¥2600
- CLOUD-CLONE CORP.(CCC,美国)
- 美国
- klRPB486Mu01
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
负20度
- 保质期：
12月
- 英文名：
Recombinant Calreticulin (CRT)
- 库存：
大量
[ PROPERTIES ] Residues: Asp199~Tyr308
Tags: N-terminal His-Tag
Accession: P14211
Host: E. coli
Subcellular Location: Endoplasmic reticulum lumen. Cytoplasmic granule. Sarcoplasmic reticulum lumen.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 4.8
Predicted Molecular Mass: 16.1kDa
Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ] Reconstitute in sterile ddH2O. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ] The sequence of the target protein is listed below. DW DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDANIYAY
[ REFERENCES ] 1. Smith M.J., Koch G.L.E. (1989) EMBO J. 8:3581-3586.
2. Mazzarella R.A., et al. (1992) Gene 120:217-225.
3. Dupuis M., et al. (1993) J. Exp. Med. 177:1-7.
4. Kozlov G., et al. (2010) J. Biol. Chem. 285:38612-38620.
Tags: N-terminal His-Tag
Accession: P14211
Host: E. coli
Subcellular Location: Endoplasmic reticulum lumen. Cytoplasmic granule. Sarcoplasmic reticulum lumen.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 4.8
Predicted Molecular Mass: 16.1kDa
Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ] Reconstitute in sterile ddH2O. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ] The sequence of the target protein is listed below. DW DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDANIYAY
[ REFERENCES ] 1. Smith M.J., Koch G.L.E. (1989) EMBO J. 8:3581-3586.
2. Mazzarella R.A., et al. (1992) Gene 120:217-225.
3. Dupuis M., et al. (1993) J. Exp. Med. 177:1-7.
4. Kozlov G., et al. (2010) J. Biol. Chem. 285:38612-38620.
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ecto-calreticulin on ER-stressed cells。 该研究发现 NKp46 能够识别外化钙网蛋白 (ecto-CRT)，在内质网应激时从内质网 (ER) 转移到细胞膜。其中，ecto-CRT 对 NKp46 的识别能够有效地控制小鼠 B16 黑色素瘤和肺癌，并增强肿瘤浸润 NK 细胞的细胞因子分泌。 图 1：来源 Nature 2. Nature：PM2.5 促进肺癌发生的机制 PM 小于 2.5 的细颗粒可以进入肺部深处，引起多种疾病。 2023 年 4 月 5 日
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钙网蛋白(CRT)重组蛋白
¥2600
