pCDNA3.0-HA-N质粒

基本信息
启动子: CMV
复制子: pUC ori，F1 ori
终止子: bGH poly(A) signal
质粒分类: 哺乳细胞真核表达载体；蛋白过表达质粒
质粒大小: 5484bp
质粒标签: N-HA
原核抗性: 氨苄青霉素(Ampicillin) 筛选标记: 新霉素(Neomycin)/遗传霉素(Geneticin/G418)
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: 哺乳细胞
诱导方式: 无需诱导
5'测序引物: T7：TAATACGACTCACTATAGGG
3'测序引物: SP6：ATTTAGGTGACACTATAGAA
备注: 载体上有起始密码子ATG，注意不要移码。
质粒简介
pCDNA3.0-HA-N载体是专门设计用来在哺乳细胞中进行高水平的稳定表达和瞬时表达。其中人巨细胞病毒早期启动子(CMV)在广泛的哺乳动物细胞中可以实现高表达。新霉素抗性基因可以用来筛选稳定细胞系，具体用G418筛选，另外HA做为标签能在WB实验中更方便的检测到目的基因是否表达。 pcDNA3.0-HA-N is 5.4 kb vectors designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells. Neomycin resistance gene for selection of stable cell lines. HA epitope for Western blot experiment.