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- KLSW
- 美国 addgene
- KL-ZL-0018
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
负20度
- 保质期：
2年
- 英文名：
PiggyBac Dual promoter (PB513B-1)
- 库存：
100
- 供应商：
kelei-bio
- 规格：
20μl
启动子: CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞，转座子载体
质粒大小: 7258bp
原核抗性: Amp
克隆菌株: DH5α
培养条件: 37℃，有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导，瞬时表达
5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物: 根据序列设计引物
质粒简介
Vectors PB510B-1 to PB514B-1 carry the CMV promoter for high expression levels in most cell types, whereas vector PB713B-1 features the MSCV promoter which is active in stem cells. The multiple cloning site (MCS) located downstream of the promoter allows for convenient cloning of your gene or microRNA of interest. Downstream of your expression cassette is an EF1alpha promoter driving the expression of either the Puro, GFP, RFP, GFP+Puro or RFP+Puro markers. The entire cassette is flanked by genomic insulator elements for stabilized expression and PiggyBac Inverted Terminal repeats for mobilization and integration
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文献和实验pGLS23 d (A, B) HIS5 CmR ADH1 promoter expresses cI followed
by Hemocytometry Counting (Sambrook and Russell 2006a ), Amplification of cDNA Generated by Reverse Transcription of mRNA (Sambrook and Russell 2006b ), Southern Blotting: Capillary Transfer of DNA to Membranes (Sambrook and Russell 2006c ), Southern
, Cas9 mediates cleavage of target DNA upstream of PAM to create a DSB within the protospacer. ( b ) Design of chimeric sgRNA for genome cleavage. The SpCas9 nuclease ( yellow ) binds to genomic DNAdirected by the sgRNA consisting of a 20-nt guide
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