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文献和实验Chemotaxis in Neutrophil-Like HL-60 Cells
known as chemotaxis. In particular, leukocyte migration in the innate immune system has been studied in the human neutrophil-like cell line (HL-60). Here, we describe the maintenance and transfection of HL-60 cells and explain how to analyze
[3H] Choline Labeling and TNF Treatment of HL-60 Cells
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room temperature PBS. 3) Resuspend final pellet in an appropriate volume of prewarmed serum free media and supplement
[3H] Choline Labeling and TNF Treatment of HL-60 Cells
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room temperature PBS. 3) Resuspend final pellet in an appropriate volume of prewarmed serum free media and supplement
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