PX396载体

PX396载体

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  • ZYbscience
  • 中国/美国
  • ZY
  • 2025年07月08日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      PX396

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    载体名称: PX396 或 PX396 Parvibaculum lavamentivorans Cas9
    质粒类型: CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体
    高拷贝/低拷贝: 高拷贝
    插入位点: 限制性内切酶，多克隆位点
    启动子: CMV
    载体大小: 10307 bp
    5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
    3' 测序引物及序列: C-CMV-24 (5'-TATTAGGACAAGGCTGGTGGGCAC-3')
    载体标签: NLS (C ter)；HA、NLS (N ter)
    载体抗性: 氨苄青霉素
    筛选标记: Neomycin
    克隆菌株: Stbl3
    宿主细胞（系）: 昆虫细胞
    备注: --
    产品目录号: ZY68333
    稳定性: 稳表达
    组成型/诱导型: 组成型
    病毒/非病毒: 杆状病毒

    载体质粒图谱和多克隆位点信息

    PX396载体图谱


    载体简介

    载体序列

    LOCUS       PX396 Parvibaculum lavamentivorans Cas9	10307 bp 	DNA	SYN
DEFINITION  PX396 Parvibaculum lavamentivorans Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="PX396 Parvibaculum lavamentivorans Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             complement(1795..2445)
                     /label="ORF frame 3"
                     /translation="MGVDSSLAVRRGSAGQPCSQCGLFIFQGLQSGCSLVSGFLLIVH
                     IRVGCIGFLQFSAFKMPVLGQMIDCPSELVSTQAFLQSPSPQFIRLVGHDHGPICRPV
                     SWQKAGFVQCLIQCLSANPSPSQLAPHHAFLSPLLVQGGPIWVPCLRENAHAQPLDVA
                     CGLGATVINHAESDTCCADVKAENPLHGRFDLALFLRRSVIWNIVWVHGGTGGISP*"
     CDS             1822..4998
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMERIFGFDIGTTSIGFSVIDYSS
                     TQSAGNIQRLGVRIFPEARDPDGTPLNQQRRQKRMMRRQLRRRRIRRKALNETLHEAG
                     FLPAYGSADWPVVMADEPYELRRRGLEEGLSAYEFGRAIYHLAQHRHFKGRELEESDT
                     PDPDVDDEKEAANERAATLKALKNEQTTLGAWLARRPPSDRKRGIHAHRNVVAEEFER
                     LWEVQSKFHPALKSEEMRARISDTIFAQRPVFWRKNTLGECRFMPGEPLCPKGSWLSQ
                     QRRMLEKLNNLAIAGGNARPLDAEERDAILSKLQQQASMSWPGVRSALKALYKQRGEP
                     GAEKSLKFNLELGGESKLLGNALEAKLADMFGPDWPAHPRKQEIRHAVHERLWAADYG
                     ETPDKKRVIILSEKDRKAHREAAANSFVADFGITGEQAAQLQALKLPTGWEPYSIPAL
                     NLFLAELEKGERFGALVNGPDWEGWRRTNFPHRNQPTGEILDKLPSPASKEERERISQ
                     LRNPTVVRTQNELRKVVNNLIGLYGKPDRIRIEVGRDVGKSKREREEIQSGIRRNEKQ
                     RKKATEDLIKNGIANPSRDDVEKWILWKEGQERCPYTGDQIGFNALFREGRYEVEHIW
                     PRSRSFDNSPRNKTLCRKDVNIEKGNRMPFEAFGHDEDRWSAIQIRLQGMVSAKGGTG
                     MSPGKVKRFLAKTMPEDFAARQLNDTRYAAKQILAQLKRLWPDMGPEAPVKVEAVTGQ
                     VTAQLRKLWTLNNILADDGEKTRADHRHHAIDALTVACTHPGMTNKLSRYWQLRDDPR
                     AEKPALTPPWDTIRADAEKAVSEIVVSHRVRKKVSGPLHKETTYGDTGTDIKTKSGTY
                     RQFVTRKKIESLSKGELDEIRDPRIKEIVAAHVAGRGGDPKKAFPPYPCVSPGGPEIR
                     KVRLTSKQQLNLMAQTGNGYADLGSNHHIAIYRLPDGKADFEIVSLFDASRRLAQRNP
                     IVQRTRADGASFVMSLAAGEAIMIPEGSKKGIWIVQGVWASGQVVLERDTDADHSTTT
                     RPMPNPILKDDAKKVSIDPIGRVRPSND*"
     CDS             complement(3273..3998)
                     /label="ORF frame 1"
                     /translation="MSGHSLFSWARICFAAYLVSFSCRAAKSSGMVLARKRLTFPGLI
                     PVPPLADTMPCSLIWMALHRSSSWPNASKGILFPFSMFTSLRHSVLFLGLLSKLRERG
                     QMCSTSYLPSLNRALKPIWSPVYGHLSWPSFHRIHFSTSSRLGLAIPFLIRSSVAFFL
                     CFSFRRMPDWISSLSLLDFPTSRPTSIRIRSGFPYSPIRLLTTFLSSFCVRTTVGLRS
                     WLMRSLSSFEAGLGSLSRISPVG*"
     CDS             complement(3316..3798)
                     /label="ORF frame 3"
                     /translation="MAKCLERHPISLLDVHVFATQCLIPWAVIKTSRARPDVFHLISA
                     FSKQGIEANLVAGVWASFLALFPQDPLLHIVPARISNSVFDQIFSGFLSLLLVPSNAR
                     LDFLPFSFGLPDVPAHFNPDSIGFSIQPDQIVDHLSQLILCSDHGWVAQLANAFPFLF
                     *"
     CDS             complement(3847..4332)
                     /label="ORF frame 3"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    complement(5031..5048)
                     /label="Sp6_primer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    5053..5077
                     /label="attB2"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     terminator      5089..5555
                     /label="hGH_PA_terminator"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     terminator      5561..5596
                     /label="T7_terminator"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     rep_origin      5713..6019
                     /label="f1_origin"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    complement(6133..6153)
                     /label="pBABE_3_primer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    complement(6139..6354)
                     /label="SV40_enhancer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     promoter        6151..6419
                     /label="SV40_promoter"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     rep_origin      6318..6395
                     /label="SV40_origin"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     misc_feature    6380..6399
                     /label="SV40pro_F_primer"
                     /translation="MQWAADFLSHPVTDHNFRHGFLSVGADGIPGWSQSWLLCSWIVA
                     QLPIPAQLVGHSRMSAGHCQSVDGMVPVICSGFLPIVSQNIVQSPQFAQLGGDLSSHC
                     FDLHWSLRSHVWPQPFQLGQDLLCSVSCVVQLPGCKILRHGLSQEAFDLSGAHPSSPF
                     G*"
     CDS             6534..7328
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6537..7325
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6843..7379)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7505..7624
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7593..7612
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7815..7850
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(7873..7890)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7880..7957
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(7987..8604)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8584..8617
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9385..10245)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9385..10245)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatggag aggattttcg gctttgacat cggcacaaca
 1921 agtatcggat tcagcgtgat tgattacagt agcacccagt ccgcaggcaa catccagagg
 1981 ctgggcgtgc gcattttccc tgaggcaagg gacccagatg ggacccccct gaaccagcag
 2041 cggagacaga aacgcatgat gaggcgccag ctgcgacgga gaaggattcg ccgaaaggca
 2101 ctgaatgaga cactgcacga agccggcttt ctgccagctt acgggtctgc agattggccc
 2161 gtggtcatgg ccgacgagcc ttatgaactg cggagaaggg gactggagga aggcctgagt
 2221 gcttacgagt tcggacgggc aatctatcat ctggcccagc accggcattt taaaggcaga
 2281 gaactggagg aatccgatac acccgaccct gatgtggacg atgagaagga agccgctaac
 2341 gagagagcag ccactctgaa ggccctgaaa aatgaacaga ccacactggg agcatggctg
 2401 gcccgccgac ccccttctga ccgcaagcga ggaatccacg cccataggaa cgtggtcgct
 2461 gaggagttcg agcgcctgtg ggaagtgcag tccaagtttc accccgctct gaaatctgag
 2521 gaaatgcggg caagaatcag tgatacaatt ttcgcccaga ggcctgtgtt ttggcgcaag
 2581 aacactctgg gagagtgcag attcatgcct ggcgaaccac tgtgtcccaa ggggtcctgg
 2641 ctgtctcagc agcggagaat gctggagaaa ctgaacaatc tggctatcgc aggcgggaat
 2701 gctaggccac tggatgcaga ggaacgcgac gccattctga gtaagctgca gcagcaggcc
 2761 agcatgtcct ggccaggcgt gcggtcagct ctgaaggcac tgtacaaaca gagaggcgag

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