| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCas9-GFP 或 pCas9_GFP |
| 质粒类型: | CRISPR/Cas9载体；基因敲除载体；哺乳动物细胞载体；双顺反子载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | CBh |
| 载体大小: | 9271 bp |
| 5' 测序引物及序列: | ggctctagtgcctctgctaacc |
| 3' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 载体标签: | T2A-GFP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | EGFP绿色荧光蛋白 |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 人胚胎干细胞 |
| 备注: | Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells |
| 产品目录号: | ZY44719 |
| 稳定性: | 瞬表达或稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCas9-GFP
- 库存：
20
- 供应商：
泽叶生物
-
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCas9_GFP 9271 bp DNA SYN DEFINITION pCas9_GFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9271 /organism="pCas9_GFP" /mol_type="other DNA" misc_feature 88..375 /label="CAG_enhancer" CDS 259..738 /label="ORF frame 1" /translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMG RGEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGE VRRQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD*" CDS complement(806..1351) /label="ORF frame 1" /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR HGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPRS PPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH KGPSRSPLRLSRSHRKETSRH*" CDS 1490..6670 /label="ORF frame 2" /translation="MGGEGLRASPRRRPLLHLQPRGCPQGDGCLRGGRGRAGFGFWRV TGGSSASANHVHAFFFFLQLLGNVLVIVLSHHFGKEFAAATMDKKYSIGLDIGTNSVG WAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRR KNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYP TIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQT YNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLT PNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILR VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDG GASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAIL RRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEV VDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAF LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDL LKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQ KGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELD INRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLL NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDE NDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPK LESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRP LIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKL IARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKN PIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNF LYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSA YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQS ITGLYETRIDLSQLGGDSRADPKKKRKVRSGGGEGRGSLLTCGDVEENPGPMVSKGEE LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTL TYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNR IELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLAD HYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK *" CDS complement(1673..2224) /label="ORF frame 1" /translation="MRQREIDQPQVSLISTVYKLLPQMIYGWVLFMVRHLVHDIAKDW VALVLFILLHQKGLLQPMEERVIHLSHLITKDLLQVADPILSAGISAPCCSFEPRGFG RLPGVEQEGANEVLLYAVAIGIAQNFEFFARHLVLVRNDGPADAVCADIEPNGVLLVH GGGREFFAKMMRQHNNQHVAQEL*" misc_feature 1686..1705 /label="pCAG_F_primer" /translation="MRQREIDQPQVSLISTVYKLLPQMIYGWVLFMVRHLVHDIAKDW VALVLFILLHQKGLLQPMEERVIHLSHLITKDLLQVADPILSAGISAPCCSFEPRGFG RLPGVEQEGANEVLLYAVAIGIAQNFEFFARHLVLVRNDGPADAVCADIEPNGVLLVH GGGREFFAKMMRQHNNQHVAQEL*" CDS complement(3149..3667) /label="ORF frame 1" /translation="MSVSFQAFFNHLPIFKQRKGEDNVLKNVLVLLIVQEVLVFNDFQ EIVIRSQGCVEAILHSADFNRVETFNLFEIVFFELFHGNFPVRLEEEVHDSFLLLSRQ ECWLSHPFCDVFDLGELVINCEVLVQQRVFRKHLFVRQIFIKVSHPFDEGLGRGPLIH DFLEVPGSDGLF*" CDS complement(3731..4264) /label="ORF frame 1" /translation="MIHVVVGEPIDVQFLIHVHVPAVLQVVQVELLILKLGVFNWVFL KDLGPQFFYTLFNPLHPFPTVLLSLLGSLVLSGHLDNDILGLMPSHYFDEFIHDLNGL QYSLFDSWATCKISDVLVKTVPLARNLCFLDVLLKGERVIMDQLHEVPVGKSIGLKKI QDCLSTLLVSDPIDQFS*" CDS complement(5258..5791) /label="ORF frame 1" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 5861..5881 /label="SV40 NLS" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 5867..5881 /label="NLS" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" gene 5954..6667 /label="EGFP" /gene="EGFP" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature complement(5996..6017) /label="EGFP_N_primer" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 6134..6163 /label="Y66 (EGFP)" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 6604..6625 /label="EGFP_C_primer" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" terminator 6716..6835 /label="SV40_PA_terminator" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 6804..6823 /label="EBV_rev_primer" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" promoter complement(6879..6897) /label="M13_reverse_primer" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature complement(6896..6918) /label="M13_pUC_rev_primer" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" promoter complement(6932..6961) /label="lac_promoter" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" gene complement(8044..8904) /label="Ampicillin" /gene="Ampicillin" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" CDS complement(8044..8904) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(8946..8974) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca 241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct 421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg 481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg 541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc 721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg 841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg 901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc 961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg 1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg 1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca 1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg 1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc 1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg 1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc 1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac 1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc 1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt 1621 gaccggcggc tctagtgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct 1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaaGAAT TCGCGGCCGC 1741 CACCATGGAC AAGAAGTACT CCATTGGGCT CGATATCGGC ACAAACAGCG TCGGCTGGGC 1801 CGTCATTACG GACGAGTACA AGGTGCCGAG CAAAAAATTC AAAGTTCTGG GCAATACCGA 1861 TCGCCACAGC ATAAAGAAGA ACCTCATTGG CGCCCTCCTG TTCGACTCCG GGGAGACGGC 1921 CGAAGCCACG CGGCTCAAAA GAACAGCACG GCGCAGATAT ACCCGCAGAA AGAATCGGAT 1981 CTGCTACCTG CAGGAGATCT TTAGTAATGA GATGGCTAAG GTGGATGACT CTTTCTTCCA 2041 TAGGCTGGAG GAGTCCTTTT TGGTGGAGGA GGATAAAAAG CACGAGCGCC ACCCAATCTT 2101 TGGCAATATC GTGGACGAGG TGGCGTACCA TGAAAAGTAC CCAACCATAT ATCATCTGAG 2161 GAAGAAGCTT GTAGACAGTA CTGATAAGGC TGACTTGCGG TTGATCTATC TCGCGCTGGC 2221 GCATATGATC AAATTTCGGG GACACTTCCT CATCGAGGGG GACCTGAACC CAGACAACAG 2281 CGATGTCGAC AAACTCTTTA TCCAACTGGT TCAGACTTAC AATCAGCTTT TCGAAGAGAA 2341 CCCGATCAAC GCATCCGGAG TTGACGCCAA AGCAATCCTG AGCGCTAGGC TGTCCAAATC 2401 CCGGCGGCTC GAAAACCTCA TCGCACAGCT CCCTGGGGAG AAGAAGAACG GCCTGTTTGG 2461 TAATCTTATC GCCCTGTCAC TCGGGCTGAC CCCCAACTTT AAATCTAACT TCGACCTGGC 2521 CGAAGATGCC AAGCTTCAAC TGAGCAAAGA CACCTACGAT GATGATCTCG ACAATCTGCT 2581 GGCCCAGATC GGCGACCAGT ACGCAGACCT TTTTTTGGCG GCAAAGAACC TGTCAGACGC 2641 CATTCTGCTG AGTGATATTC TGCGAGTGAA CACGGAGATC ACCAAAGCTC CGCTGAGCGC 2701 TAGTATGATC AAGCGCTATG ATGAGCACCA CCAAGACTTG ACTTTGCTGA AGGCCCTTGT 2761 CAGACAGCAA CTGCCTGAGA AGTACAAGGA AATTTTCTTC GATCAGTCTA AAAATGGCTA 2821 CGCCGGATAC ATTGACGGCG GAGCAAGCCA GGAGGAATTT TACAAATTTA TTAAGCCCAT 2881 CTTGGAAAAA ATGGACGGCA CCGAGGAGCT GCTGGTAAAG CTTAACAGAG AAGATCTGTT 2941 GCGCAAACAG CGCACTTTCG ACAATGGAAG CATCCCCCAC CAGATTCACC TGGGCGAACT 3001 GCACGCTATC CTCAGGCGGC AAGAGGATTT CTACCCCTTT TTGAAAGATA ACAGGGAAAA 3061 GATTGAGAAA ATCCTCACAT TTCGGATACC CTACTATGTA GGCCCCCTCG CCCGGGGAAA 3121 TTCCAGATTC GCGTGGATGA CTCGCAAATC AGAAGAGACC ATCACTCCCT GGAACTTCGA 3181 GGAAGTCGTG GATAAGGGGG CCTCTGCCCA GTCCTTCATC GAAAGGATGA CTAACTTTGA 3241 TAAAAATCTG CCTAACGAAA AGGTGCTTCC TAAACACTCT CTGCTGTACG AGTACTTCAC 3301 AGTTTATAAC GAGCTCACCA AGGTCAAATA CGTCACAGAA GGGATGAGAA AGCCAGCATT 3361 CCTGTCTGGA GAGCAGAAGA AAGCTATCGT GGACCTCCTC TTCAAGACGA ACCGGAAAGT 3421 TACCGTGAAA CAGCTCAAAG AAGACTATTT CAAAAAGATT GAATGTTTCG ACTCTGTTGA 3481 AATCAGCGGA GTGGAGGATC GCTTCAACGC ATCCCTGGGA ACGTATCACG ATCTCCTGAA 3541 AATCATTAAA GACAAGGACT TCCTGGACAA TGAGGAGAAC GAGGACATTC TTGAGGACAT 3601 TGTCCTCACC CTTACGTTGT TTGAAGATAG GGAGATGATT GAAGAACGCT TGAAAACTTA 3661 CGCTCATCTC TTCGACGACA AAGTCATGAA ACAGCTCAAG AGGCGCCGAT ATACAGGATG 3721 GGGGCGGCTG TCAAGAAAAC TGATCAATGG GATCCGAGAC AAGCAGAGTG GAAAGACAAT 3781 CCTGGATTTT CTTAAGTCCG ATGGATTTGC CAACCGGAAC TTCATGCAGT TGATCCATGA 3841 TGACTCTCTC ACCTTTAAGG AGGACATCCA GAAAGCACAA GTTTCTGGCC AGGGGGACAG 3901 TCTTCACGAG CACATCGCTA ATCTTGCAGG TAGCCCAGCT ATCAAAAAGG GAATACTGCA 3961 GACCGTTAAG GTCGTGGATG AACTCGTCAA AGTAATGGGA AGGCATAAGC CCGAGAATAT 4021 CGTTATCGAG ATGGCCCGAG AGAACCAAAC TACCCAGAAG GGACAGAAGA ACAGTAGGGA 4081 AAGGATGAAG AGGATTGAAG AGGGTATAAA AGAACTGGGG TCCCAAATCC TTAAGGAACA 4141 CCCAGTTGAA AACACCCAGC TTCAGAATGA GAAGCTCTAC CTGTACTACC TGCAGAACGG 4201 CAGGGACATG TACGTGGATC AGGAACTGGA CATCAATCGG CTCTCCGACT ACGACGTGGA 4261 TCATATCGTG CCCCAGTCTT TTCTCAAAGA TGATTCTATT GATAATAAAG TGTTGACAAG 4321 ATCCGATAAA AATAGAGGGA AGAGTGATAA CGTCCCCTCA GAAGAAGTTG TCAAGAAAAT 4381 GAAAAATTAT TGGCGGCAGC TGCTGAACGC CAAACTGATC ACACAACGGA AGTTCGATAA 4441 TCTGACTAAG GCTGAACGAG GTGGCCTGTC TGAGTTGGAT AAAGCCGGCT TCATCAAAAG 4501 GCAGCTTGTT GAGACACGCC AGATCACCAA GCACGTGGCC CAAATTCTCG ATTCACGCAT 4561 GAACACCAAG TACGATGAAA ATGACAAACT GATTCGAGAG GTGAAAGTTA TTACTCTGAA 4621 GTCTAAGCTG GTCTCAGATT TCAGAAAGGA CTTTCAGTTT TATAAGGTGA GAGAGATCAA 4681 CAATTACCAC CATGCGCATG ATGCCTACCT GAATGCAGTG GTAGGCACTG CACTTATCAA 4741 AAAATATCCC AAGCTTGAAT CTGAATTTGT TTACGGAGAC TATAAAGTGT ACGATGTTAG 4801 GAAAATGATC GCAAAGTCTG AGCAGGAAAT AGGCAAGGCC ACCGCTAAGT ACTTCTTTTA 4861 CAGCAATATT ATGAATTTTT TCAAGACCGA GATTACACTG GCCAATGGAG AGATTCGGAA 4921 GCGACCACTT ATCGAAACAA ACGGAGAAAC AGGAGAAATC GTGTGGGACA AGGGTAGGGA 4981 TTTCGCGACA GTCCGGAAGG TCCTGTCCAT GCCGCAGGTG AACATCGTTA AAAAGACCGA 5041 AGTACAGACC GGAGGCTTCT CCAAGGAAAG TATCCTCCCG AAAAGGAACA GCGACAAGCT 5101 GATCGCACGC AAAAAAGATT GGGACCCCAA GAAATACGGC GGATTCGATT CTCCTACAGT 5161 CGCTTACAGT GTACTGGTTG TGGCCAAAGT GGAGAAAGGG AAGTCTAAAA AACTCAAAAG 5221 CGTCAAGGAA CTGCTGGGCA TCACAATCAT GGAGCGATCA AGCTTCGAAA AAAACCCCAT 5281 CGACTTTCTC GAGGCGAAAG GATATAAAGA GGTCAAAAAA GACCTCATCA TTAAGCTTCC 5341 CAAGTACTCT CTCTTTGAGC TTGAAAACGG CCGGAAACGA ATGCTCGCTA GTGCGGGCGA 5401 GCTGCAGAAA GGTAACGAGC TGGCACTGCC CTCTAAATAC GTTAATTTCT TGTATCTGGC 5461 CAGCCACTAT GAAAAGCTCA AAGGGTCTCC CGAAGATAAT GAGCAGAAGC AGCTGTTCGT 5521 GGAACAACAC AAACACTACC TTGATGAGAT CATCGAGCAA ATAAGCGAAT TCTCCAAAAG 5581 AGTGATCCTC GCCGACGCTA ACCTCGATAA GGTGCTTTCT GCTTACAATA AGCACAGGGA 5641 TAAGCCCATC AGGGAGCAGG CAGAAAACAT TATCCACTTG TTTACTCTGA CCAACTTGGG 5701 CGCGCCTGCA GCCTTCAAGT ACTTCGACAC CACCATAGAC AGAAAGCGGT ACACCTCTAC 5761 AAAGGAGGTC CTGGACGCCA CACTGATTCA TCAGTCAATT ACGGGGCTCT ATGAAACAAG 5821 AATCGACCTC TCTCAGCTCG GTGGAGACAG CAGGGCTGAC CCCAAGAAGA AGAGGAAGGT 5881 GAGGTCCGGC GGCGGAGAGG GCAGAGGAAG TCTTCTAACA TGCGGTGACG TGGAGGAGAA 5941 TCCCGGCCCA ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 6001 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA 6061 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 6121 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA 6181 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG 6241 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 6301 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT 6361 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA 6421 GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT 6481 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC 6541 CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA 6601 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT 6661 GTACAAGTAA ctgcagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 6721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6841 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 6901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6961 agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8041 cagttaCCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 8101 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 8161 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 8221 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 8281 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 8341 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 8401 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 8461 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 8521 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 8581 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 8641 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 8701 GCTCATcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc 9061 tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca 9121 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc 9181 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt 9241 tgcgctgctt cgcgatgtac gggccagata t //
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文献和实验相关实验
doctorchihlee 大家好，请问一下空白载体（含GFP,但不含靶基因）成功转染哺乳动物细胞后，荧光显微镜下观察GFP，观察到细胞全部呈绿色还是仅仅外周发光或是核发光？谢谢！ doctorchihlee GFP在细胞质，是全部发光？！？ real_madrid_146 这个是我用pEGFP-C2转HEK293的图。 shylook 就是这样的
【求助】请问是否有带GFP的表达载体可以用了表达microRNA
xshdao 因课题需要开展microRNA表达载体构建，查了一些文献好像很少有带GFP报告基因的microRNA表达载体，如pCDNA3.1。请问是否有带GFP的表达载体可以用了表达microRNA？请各位高手发表高见！ freecell google: microrna expression vector with gfp wanghm200810 有这样的载体，还有带抗性基因的载体
【求助】怎样判断在一个细胞中同时了导入两个含GFP标记的慢病毒过表达载体？感谢高手指点！
xieguozhu 在下欲构建两个含GFP的慢病毒过表达载体，但是有一个关键问题就是我构建的这两个目的基因是要导入同一个细胞的，我要表达的目的基因是miR-141, miR-200c. 要感染的细胞是MDA-MB-231， 这两种表达载体都是GFP标记的，我不知道在最后怎样判断两个基因都导入细胞了，后来我在想可不可以在同一个病毒上同时插入两个目的基因，比如miR-200c/miR-141(因为这两个基因在同一条染色体上，且靠的很近，中间隔了不到400个碱基
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pCas9-GFP载体
¥1000
