pcambia1302载体

LOCUS       AF234298               10549 bp    DNA     circular SYN 24-APR-2000
DEFINITION  Binary vector pCAMBIA-1302, complete sequence.
ACCESSION   AF234298
VERSION     AF234298.1  GI:7638073
KEYWORDS    .
SOURCE      Binary vector pCAMBIA-1302
  ORGANISM  Binary vector pCAMBIA-1302
            other sequences; artificial sequences; vectors.
REFERENCE   1  (sites)
  AUTHORS   Hajdukiewicz,P., Svab,Z. and Maliga,P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors
            for plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
   PUBMED   7919218
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     A comprehensive set of modular vectors for advanced manipulations
            and efficient transformation of plants
  JOURNAL   Unpublished
  REMARK    Full description of constructs
REFERENCE   3  (bases 1 to 10549)
  AUTHORS   Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
            Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
            Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
            Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
            GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Binary vector pCAMBIA-1302"
                     /mol_type="other DNA"
                     /db_xref="taxon:118390"
     CDS             2..757
                     /note="mgfp5* His6"
                     /codon_start=1
                     /product="mGFP5*, hexaHis tagged"
                     /protein_id="AAF65344.1"
                     /db_xref="GI:7638075"
                     /translation="MVDLTSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTI
                     FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK
                     QKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKASHHHHHHV"
     misc_feature    786..1038
                     /note="nos (nopaline synthase) 3'UTR (polyA signal)"
     misc_feature    1076..1101
                     /note="right border T-DNA repeat"
     misc_feature    complement(2142..3142)
                     /note="STA region from pVS1 plasmid"
     rep_origin      complement(3735..4735)
                     /note="pVS1-REP; replication origin from pVS1"
     misc_feature    complement(5145..5405)
                     /note="bom site from pBR322"
     rep_origin      complement(5545..5825)
                     /note="pBR322 origin of replication"
     CDS             complement(6116..6910)
                     /note="aadA (kanamycin resistance) gene amplified from
                     pIG121Hm"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="AAF65345.1"
                     /db_xref="GI:7638076"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7335..7360
                     /note="left border repeat from C58 T-DNA"
     misc_feature    7427..7635
                     /note="CaMV 3'UTR (polyA signal)"
     CDS             complement(7651..8676)
                     /note="hptII (hygromycin resistance) gene"
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /protein_id="AAF65346.1"
                     /db_xref="GI:7638077"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKK"
     promoter        complement(8712..9492)
                     /note="CaMV35S2; CaMV 35S promoter, duplicated"
     promoter        9640..9694
                     /note="PlacZ; lacZ promoter"
     CDS             9722..9955
                     /codon_start=1
                     /product="LacZ alpha fragment"
                     /protein_id="AAF65343.1"
                     /db_xref="GI:7638074"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
                     RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    9736..9792
                     /note="pUC18 MCS; polylinker"
     promoter        10006..10543
                     /note="CaMV35S; 35S promoter from CaMV"
ORIGIN      
        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc
      781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
      841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
      901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
      961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
     1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     3421 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
     3481 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     3541 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     3601 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     3661 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     3721 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     3781 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     3841 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     3901 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     3961 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca