企业认证
-20℃低温保存
三年
pPZP202
20
泽叶生物
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pPZP202 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 7132 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward：CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | 无 |
| 载体抗性: | 壮观霉素或链霉素 |
| 筛选标记: | -- |
| 克隆菌株: | HB101 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | 重组质粒可用蓝白斑法筛选 |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
LOCUS pPZP202 7132 bp DNA SYN
DEFINITION pPZP202
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7132
/organism="pPZP202"
/mol_type="other DNA"
CDS 202..888
/label="ORF frame 1"
/translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITG
TRRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWA
DTTTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHAR
ELIDQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAA
AENERQEEQA*"
CDS 1187..1816
/label="ORF frame 2"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
CDS complement(2091..2684)
/label="ORF frame 3"
/translation="MGNRHQYQVVIPHTGHAGRPCGNLYVPVWKLVADHLASSSVTLR
QTENGHVHDAATIAGAHVIEHRNEKIWLLVALGRLPNRRRTGCRRLPGFFADSISGRL
PRFTGACFCLDALPLGGLRGLQLLHQVITQRRADLYRAGWFATVTPIPRAWGFQCHCR
AGRQPSRLRLANRPFLHTWGIPRRRCLVVLDFPCRLL*"
CDS 2211..3317
/label="ORF frame 3"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
misc_feature complement(3813..3835)
/label="pGEX_3_primer"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
rep_origin complement(4232..4851)
/label="pBR322_origin"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
gene complement(5079..6078)
/label="spect"
/gene="spect"
/translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
CDS complement(5079..6011)
/label="ORF frame 3"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
misc_feature complement(6508..6651)
/label="lacZ_a"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
misc_feature 6622..6644
/label="M13_pUC_fwd_primer"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
promoter 6637..6653
/label="M13_forward20_primer"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
promoter complement(6719..6737)
/label="M13_reverse_primer"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
misc_feature complement(6736..6758)
/label="M13_pUC_rev_primer"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
promoter complement(6772..6801)
/label="lac_promoter"
/translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
LNIMGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
AYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK*"
ORIGIN
1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
6481 acgccgaatt gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
6541 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
6601 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
6661 ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca
6721 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
6781 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
6841 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
6901 atcggccaac gcgcggggag aggcggtttg cgtattggag cttgagcttg gatcagattg
6961 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
7021 taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc
7081 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aa
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Western免疫印迹（Western Blot）是将蛋白质转移到膜上，然后利用抗体进行检测。对已知表达蛋白，可用相应抗体作为一抗进行检测，对新基因的表达产物，可通过融合部分的抗体检测。一、原理与Southern或Northern杂交方法类似，但Western Blot采用的是聚丙烯酰胺凝胶电泳，被检测物是蛋白质，“探针”是抗体，“显色”用标记的二抗。经过PAGE分离的蛋白质样品，转移到固相载体（例如硝酸纤维素薄膜）上，固相载体以非共价键形式吸附蛋白质，且能保持电泳分离的多肽类型及其生物学活性
烯酰胺凝胶电泳，被检测物是蛋白质，「探针」是抗体，「显色」用标记的二抗。经过 PAGE 分离的蛋白质样品，转移到固相载体（例如硝酸纤维素薄膜）上，固相载体以非共价键形式吸附蛋白质，且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原，与对应的抗体起免疫反应，再与酶或同位素标记的第二抗体起反应，经过底物显色或放射自显影以检测电泳分离的特异性目的基因表达的蛋白成分。该技术也广泛应用于检测蛋白水平的表达。实验步骤一、试剂准备1. SDS-PAGE 试剂：见聚丙烯酰胺凝胶电泳实验。2. 匀浆
金黄色葡萄球菌A蛋白（staphylococcal protein A，SPA）可与IC中IgG的Fc段结合。将待测血清用低浓度PEG沉淀后加至SPA包被的固相载体上，再以酶标记的SPA与之反应，即可检测样本中有无IC。1、试剂（1）5%与2.5%PEG用0.02mol/LpH7.4PBS配制；（2）牛血清白蛋白（BSA）缓冲液：用0.05mol/L pH 7.4PBS配制。含0.01mol/L EDTA、0.1%硫柳汞、0.05%Tween 20及4%BSA；（3）HRP-SPA用改良过碘酸
技术资料暂无技术资料 索取技术资料
