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pET-41c(+)载体

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  • ¥800
  • ZYbscience
  • 中国/美国
  • ZY70558-3
  • 2025年07月15日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pET-41c(+)

    • 库存：

      20

    • 供应商：

      泽叶生物

    pET41c载体基本信息

    出品公司: ZYbscience
    别名: pET41c, pET 41c
    质粒类型: 大肠杆菌蛋白表达
    表达水平: 高
    克隆方法: 多克隆位点，限制性内切酶
    载体大小: 5934 bp
    5' 测序引物: T7或pGEX-5‘
    5' 测序引物序列: T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
    载体标签: N-GST, N-His, N-Thrombin
    载体抗性: Kanamycin
    备注:
    Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; PshAI blunt cloning site; a,b,c vary by MCS
    产品目录号: ZY70558-3
    稳定性: 瞬时表达 Transient
    组成型: 组成型 Constitutive
    病毒/非病毒: 非病毒

    pET41c载体质粒图谱和多克隆位点信息

    pet41c载体图谱
    pet41c多克隆位点
    pet41c载体克隆方法说明

    Feature Name Start End
    T7_terminator 120 1
    T7_Terminal_primer 69 87
    EK 280 266
    S15 355 311
    6xHIS 415 398
    GST (variant) 1096 446
    T7_transl_en_RBS 1121 1105
    lacO 1166 1139
    T7_promoter 1184 1166
    tet (300 - 563) 1220 1483
    pBRrevBam_primer 1291 1272
    lacI 1566 2657
    ROP 3229 3420
    pGEX_3_primer 3436 3414
    pBR322_origin 4454 3835
    KanR2 4560 5375
    ORF Start End
    ORF frame 3 1096 143
    ORF frame 2 1097 1825
    ORF frame 3 1698 2657
    ORF frame 3 4560 5375
    Enzyme Name Cut
    XhoI 174
    NotI 182
    EagI 182
    HindIII 189
    SalI 195
    SacI 206
    PstI 214
    AscI 216
    StuI 226
    EcoRI 235
    BamHI 241
    EcoRV 251
    NcoI 256
    AgeI 293
    KpnI 300
    BglII 303
    SacII 394
    SpeI 425
    MscI 888
    NdeI 1095
    XbaI 1133
    ApaI 2136
    HpaI 2431
    NruI 4648
    SmaI 4865
    XmaI 4863

    pET41c载体简介

    The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Therefore, single stranded sequencing should be performed using the T7 terminator primer (cat. no. 69337-3).Vector encoded sequence can be completely removed when cloning into the PshAI site (as shown below) and then cleaving the GST fusion protein with Enterokinase.

    pET41c载体序列

    LOCUS pET-41 c(+) 5934 bp DNA Circular SYN
    DEFINITION pET-41 c(+)
    ACCESSION
    KEYWORDS
    SOURCE
    ORGANISM other sequences; artificial sequences; vectors.
    COMMENT This file is created by Vector NTI
    http://www.biofeng.com/
    COMMENT ORIGDB|GenBank
    COMMENT VNTAUTHORNAME|biofeng.com|
    COMMENT VNTOAUTHORAD1|Novagen Inc.|
    FEATURES Location/Qualifiers
    source 1..5934
    /organism="pET-41 c(+)"
    /mol_type="other DNA"
    terminator complement(1..120)
    /label="T7_terminator"
    misc_feature 69..87
    /label="T7_Terminal_primer"
    CDS complement(143..1096)
    /label="ORF frame 3"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
    misc_feature complement(266..280)
    /label="EK"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
    misc_feature complement(311..355)
    /label="S15"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
    misc_feature complement(398..415)
    /label="6xHIS"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
    gene complement(446..1096)
    /label="GST (variant)"
    /gene="GST (variant)"
    /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
    KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI
    RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALD
    VVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
    SDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDD
    KSPMAISGIRILYRPWRACRRAPSTSLRPHSSTTTTTTTTN*"
    CDS 1097..1825
    /label="ORF frame 2"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(1105..1121)
    /label="T7_transl_en_RBS"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(1139..1166)
    /label="lacO"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    promoter complement(1166..1184)
    /label="T7_promoter"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    gene 1220..1483
    /label="tet (300 - 563)"
    /gene="tet (300 - 563)"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature complement(1272..1291)
    /label="pBRrevBam_primer"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    misc_feature 1566..2657
    /label="lacI"
    /translation="MYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSISILYAGR
    IVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHA
    PFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGEC
    ETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQ
    TVVADWRCHLQSGPARAVANCRGD*"
    CDS 1698..2657
    /label="ORF frame 3"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature 3229..3420
    /label="ROP"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    misc_feature complement(3414..3436)
    /label="pGEX_3_primer"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    rep_origin complement(3835..4454)
    /label="pBR322_origin"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    gene 4560..5375
    /label="KanR2"
    /gene="KanR2"
    /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
    RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
    PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
    HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
    SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
    LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
    CDS 4560..5375
    /label="ORF frame 3"
    /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
    GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
    GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
    VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
    RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
    ORIGIN
    1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
    61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
    121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
    181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
    241 ggatccccga tatcgccatg ggactcttgt cgtcgtcatc accggagcca ccaccggtac
    301 ccagatctgg gctgtccatg tgctggcgtt cgaatttagc agcagcggtt tctttcatac
    361 caattgcagt actaccgcgt ggcaccagac ccgcggagtg atggtgatgg tgatgaccag
    421 aaccactagt tgaaccatcc gattttggag gatggtcgcc accaccaaac gtggcttgcc
    481 agccctgcaa aggccatgct atatacttgc tggatttcaa gtacttatca atttgtggga
    541 tagcttcaat acgtttttta aaacaaacta attttgggaa cgcatccagg cacattgggt
    601 ccatgtataa aacaacatca agagcgtcat acaacatgaa gtcaggatgg gttacatgat
    661 caccatttaa atatgtttta tgacataaac gatcttcgaa cattttcagc atttcaggta
    721 gcttgctaag aaaatcaact ttgagagttt caaagtcttt actatatgca attctcgaaa
    781 caccgtatct aatatccaaa accgctcctt caagcattga aatctctgca cgctcttttg
    841 gacaaccacc caacatgttg tgcttgtcag ctatataacg tatgatggcc atagactgtg
    901 ttaatttaac atcaccatca atataataag gaagattggg aaactccaaa cccaattcaa
    961 actttttgtt tcgccattta tcaccttcat cgcgctcata caaatgctct tcatattttt
    1021 cttcaagata ttccaaaaga agtcgagtgg gttgcacaag gcccttaatt ttccaataac
    1081 ctagtatagg ggacatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
    1141 ggaattgtta tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag
    1201 atcgatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
    1261 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
    1321 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
    1381 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
    1441 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca
    1501 ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
    1561 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
    1621 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
    1681 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
    1741 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
    1801 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
    1861 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
    1921 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
    1981 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
    2041 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
    2101 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
    2161 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac

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