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pLKO-DEST-YFP载体

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  • ZYbscience
  • 中国/美国
  • ZY32683
  • 2025年07月14日
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    上海泽叶生物科技有限公司
    11 年钻石会员

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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pLKO-DEST-YFP

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    载体名称: pLKO-DEST-YFP 或 pLKO.DEST.YFP
    质粒类型: 慢病毒载体；RNAi载体；cDNA/shRNA gateway
    高拷贝/低拷贝: 低拷贝
    克隆方法: gateway
    启动子: U6
    载体大小: 8897 bp
    5' 测序引物及序列: --
    3' 测序引物及序列: --
    载体标签: --
    载体抗性: 氨苄青霉素、氯霉素
    筛选标记: YFP
    克隆菌株: 阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞，以维持质粒的稳定性。
    宿主细胞（系）: 哺乳动物细胞
    备注:
    其
    产品目录号: ZY32683
    稳定性: 稳表达
    组成型/诱导型: 组成型
    病毒/非病毒: 慢病毒

    载体质粒图谱和多克隆位点信息

    pLKO-DEST-YFP载体图谱


    载体简介

    载体序列

    LOCUS       pLKO.DEST.YFP	8897 bp 	DNA	SYN
DEFINITION  pLKO.DEST.YFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="pLKO.DEST.YFP"
                     /mol_type="other DNA"
     misc_feature    complement(114..221)
                     /label="attR1"
     misc_feature    114..214
                     /label="attR2"
     misc_feature    complement(300..323)
                     /label="MSCV_rev_primer"
     misc_feature    704..723
                     /label="mPGK_F_primer"
     CDS             796..1689
                     /label="ORF frame 1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPAIGSLVDRITDLS
                     PQGDPGRHHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLRQADPEVHLH
                     HRQAARALAHPRDHLRLRPAVLRPLPRPHEAARLLQVRHARRLRPGAHHLLQGRRQLQ
                     DPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAEERHQGEL
                     QDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPELPVRPEQRPQREARSHGPAGV
                     RDRRRDHSRHGRAVQVKRPRL*"
     CDS             953..1672
                     /label="ORF frame 2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            956..1663
                     /label="EYFP"
                     /gene="EYFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(998..1019)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    1544..1582
                     /label="T203Y (EYFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    1606..1627
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2022..2037
                     /label="cPPT"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2022..2043
                     /label="U3PPT"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2039..2091
                     /label="delta_U3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2092..2272
                     /label="HIV-1_5_LTR"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2092..2272
                     /label="truncHIV-1_3_LTR"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     terminator      2347..2466
                     /label="SV40_PA_terminator"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2435..2454
                     /label="EBV_rev_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      2484..2582
                     /label="SV40_origin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2567..2586
                     /label="SV40pro_F_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     rep_origin      2798..3104
                     /label="f1_origin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        3297..3325
                     /label="AmpR_promoter"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            3367..4227
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             3367..4227
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4347..4931
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5242..5260
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5277..5296
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5550..5730
                     /label="truncHIV-1_3_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5550..5730
                     /label="HIV-1_5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5841..5885
                     /label="HIV-1_psi_pack"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             6273..6872
                     /label="ORF frame 3"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    6395..6628
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     promoter        7165..7397
                     /label="hU6_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    7327..7346
                     /label="LKO1_5_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    7431..7555
                     /label="attR1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     misc_feature    complement(7455..7555)
                     /label="attR2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     promoter        7580..7603
                     /label="lac_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     gene            7664..8323
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWV*"
     CDS             7664..8323
                     /label="ORF frame 2"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    8665..73
                     /label="ccdB"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
ORIGIN
    1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
   61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
  121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
  181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
  241 atgggcggcc gctctagagg gcccaagctt acgaggcgct tttcccaagg cagtctggag
  301 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
  361 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
  421 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
  481 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
  541 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
  601 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
  661 gcggggcggg cgcccgaagg tcctccggag gcccggtgtt ccgcattctg cacgcttcaa
  721 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
  781 ccaagctagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
  841 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc catcggctcc
  901 ctcgttgacc gaatcaccga cctctctccc cagggggatc ccggtcgcca ccatggtgag
  961 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
 1021 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
 1081 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
 1141 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
 1201 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
 1261 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
 1321 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
 1381 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
 1441 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
 1501 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
 1561 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
 1621 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
 1681 cgactctagc atgtttaagg gttccggttc cactaggtac aattcgatat caagcttatc
 1741 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
 1801 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
 1861 cgtatggctt tcattttctc ctccttgacc ggagcttacc cgcccgcccc acgacccgca
 1921 gcgcccgacc gaaaggagcg cacgacccca tgcatcgtta agagctcggt acctttaaga
 1981 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
 2041 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
 2101 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
 2161 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
 2221 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
 2281 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
 2341 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
 2401 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
 2461 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
 2521 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
 2581 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
 2641 tagggacgta cccaattcgc ctggcgttac ccaacttaat cgccttgcag cacatccccc
 2701 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
 2761 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
 2821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
 2881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
 2941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
 3001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
 3061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
 3121 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
 3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
 3241 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
 3301 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
 3361 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
 3421 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
 3481 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
 3541 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
 3601 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
 3661 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
 3721 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
 3781 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
 3841 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
 3901 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
 3961 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
 4021 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
 4081 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
 4141 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
 4201 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
 4261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
 4321 ctcatgagga agcggaagag cgctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
 4381 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaaggtaa
 4441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
 4501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
 4561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
 4621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
 4681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
 4741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
 4801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
 4861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
 4921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
 4981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
 5041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
 5101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
 5161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
 5221 ctcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt

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