| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pENTR-GUS |
| 质粒类型: | Gateway载体；入门载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | 无 |
| 克隆方法: | 限制性内切酶；多克隆位点 |
| 载体大小: | 3841 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素 |
| 真核筛选标记: | 无 |
| 克隆菌株: | DH5a 、TOP10感受态细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 病毒/非病毒: | 非病毒 |
- ¥2000
- ZYbscience
- 中国/美国
- ZY
- 2025年07月12日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pENTR-GUS
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 3841 bp ds-DNA circular SYN 13-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS pENTR-GUS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3841)
AUTHORS Biofeng Lab.
FEATURES Location/Qualifiers
source 1..3841
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 99..198
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL1"
/note="recombination site for the Gateway(R) LR reaction"
CDS 228..2039
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/note="GUS"
/translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
protein_bind complement(2041..2140)
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL2"
/note="recombination site for the Gateway(R) LR reaction"
rep_origin complement(2259..2847)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2990..3805)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
1 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
61 cacgggccag agctgcagct agcatggatc tcgggcccca aataatgatt ttattttgac
121 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
181 tgtacaaaaa agcaggctca tttaacttta agaaggagat atataccatg gtccgtcctg
241 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc
301 gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa
361 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg
421 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg
481 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga
541 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg
601 ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc
661 cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct
721 ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg
781 acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc
841 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg
901 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg
961 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct
1021 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc
1081 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggactta cgtggcaaag
1141 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact
1201 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg
1261 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt
1321 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc
1381 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg
1441 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt
1501 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca
1561 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc
1621 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac
1681 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat
1741 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt
1801 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg
1861 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg
1921 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc
1981 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaataat
2041 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
2101 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct agcggtaata
2161 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
2221 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
2281 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
2341 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
2401 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
2461 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
2521 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
2581 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
2641 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
2701 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
2761 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
2821 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
2881 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagcttgcg ccgtcccgtc
2941 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
3001 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
3061 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
3121 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
3181 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
3241 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
3301 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
3361 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
3421 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
3481 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
3541 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
3601 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
3661 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
3721 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag
3781 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
3841 g
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pENTR-GUS载体
¥2000
