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- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
三年
- 英文名:
pGL4.37[luc2P/ARE/Hygro]
- 库存:
60
- 供应商:
信裕生物
- 规格:
5ug质粒
基本信息
| 质粒类型: | 启动子报告载体 |
|---|---|
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 6086 bp |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: | Promoterless vector encoding destabilized luciferase for measuring the activity of promoter and enhancer sequences. |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1565 | pGL4.37[luc2P/ARE/Hygro] | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
The pGL4.37[luc2P/ARE/Hygro] Vector(a–e) contains four copies of an antioxidant response element (ARE) that drives transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. Theluc2P gene contains hPEST, a protein destabilization sequence, which allows luc2P protein levels to respond more quickly than those of luc2 to induction of transcription. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a gene for hygromycin resistance to allow selection of stably transfected mammalian cell lines.
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国自然/高分文章利器!CRISPR文库筛选保姆级指南:从Cas9递送到表型选择一文打通!
-1 2、靶向E2F8通过破坏RRM2驱动的DNA修复增敏吉西他滨耐药的胆囊癌对PARP抑制剂的疗效 发表期刊:Journal of Experimental & Clinical Cancer Research 研究机构:复旦大学附属中山医院 研究人员在吉西他滨耐药的NOZ-R胆囊癌细胞中进行了全基因组CRISPR ko筛选(GeCKO v2文库)。敲除转录因子E2F8可显著增强耐药细胞对PARP抑制剂奥拉帕尼的敏感性。机制上,E2F8通过转录上调RRM
() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
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