- ¥1000
- XYbscience
- 中国/美国
- XY1565
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
三年
- 英文名：
pGL4.37[luc2P/ARE/Hygro]
- 库存：
60
- 供应商：
信裕生物
- 规格：
5ug质粒
基本信息
| 质粒类型: | 启动子报告载体 |
|---|---|
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6086 bp |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: | Promoterless vector encoding destabilized luciferase for measuring the activity of promoter and enhancer sequences. |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1565 | pGL4.37[luc2P/ARE/Hygro] | 5ug质粒 |
¥1000.00 |
质粒图谱
![pGL4.37[luc2P/ARE/Hygro] 质粒图谱](https://gz.yonghuischool.com/sites/default/files/styles/large/public/product/images/vector/pGL4.37.png?itok=e0TzCYIa)
![pGL4.37[luc2P/ARE/Hygro] 载体图谱](https://gz.yonghuischool.com/sites/default/files/styles/large/public/product/images/vector/pGL4.37_luc2P_ARE_Hygro_1x.png?itok=dyhDMplV)
载体描述
The pGL4.37[luc2P/ARE/Hygro] Vector(a–e) contains four copies of an antioxidant response element (ARE) that drives transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. Theluc2P gene contains hPEST, a protein destabilization sequence, which allows luc2P protein levels to respond more quickly than those of luc2 to induction of transcription. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a gene for hygromycin resistance to allow selection of stably transfected mammalian cell lines.
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文献和实验a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
附录转化部分。 抗性: Amp; 37℃，培养过夜 转化后X基因分别平板挑菌, 37℃ 250转/分钟摇菌14小时，PCR鉴定后，将阳性菌液送上海权阳生物技术有限公司测序。 X基因慢病毒载体单克隆菌落PCR鉴定（使用载体通用引物,PCR条带大小应比实际大200bp左右）：
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