| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pYES3-CT, pYES3/CT |
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5870 bp |
| 5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC |
| 3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
| 载体标签: | C-His, C-V5 |
| 载体抗性: | 氨苄 |
| 筛选标记: | TRP1 |
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
| 产品目录号: | ZY8253-20 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
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- 中国/美国
- ZY8253-20
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pYES3/CT
- 库存：
20
- 供应商：
泽叶生物
pYES3-CT载体基本信息
pYES3-CT载体质粒图谱和多克隆位点信息
pYES3-CT载体简介
pYES3-CT载体序列
LOCUS pYES3/CT 5870 bp DNA circular SYN
DEFINITION pYES3/CT
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5870
/organism="pYES3/CT"
/mol_type="other DNA"
promoter 1..451
/label="GAL1_promoter"
misc_feature 414..437
/label="GAL1_primer"
promoter 475..493
/label="T7_promoter"
terminator 716..961
/label="CYC1_terminator"
misc_feature complement(725..743)
/label="CYC1_primer"
misc_feature complement(725..743)
/label="pYESTrp_rev_primer"
gene complement(1963..2823)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1963..2823)
/label="ORF frame 3"
promoter complement(2865..2893)
/label="AmpR_promoter"
gene 3133..3807
/label="TRP1"
/gene="TRP1"
CDS 3133..3807
/label="ORF frame 1"
rep_origin 3859..5330
/label="2micron_origin"
ORIGIN
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
541 AGTGTGCTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
601 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
661 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCCTAGA GGGCCGCATC ATGTAATTAG
721 TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG
781 TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA
841 CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA
901 TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTGCAAGC
961 TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
1141 GAACATGTGA GCAAAAGGCC AGCAAAAGCC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
2041 CGTGTAGATA ACTACGATAC GGGAGCGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC
2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
2821 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
2881 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
2941 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
3001 GCGTATCACG AGGCCCTTTC GTCTTCAAGA AATTCGGTCG AAAAAAGAAA AGGAGAGGGC
3061 CAAGAGGGAG GGCATTGGTG ACTATTGAGC ACGTGAGTAT ACGTGATTAA GCACACAAAG
3121 GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT CTGGTCCATT GGTGAAAGTT
3181 TGCGGCTTGC AGAGCACAGA GGCCGCAGAA TGTGCTCTAG ATTCCGATGC TGACTTGCTG
3241 GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC CGGTTATTGC AAGGAAAATT
3301 TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC CGAAATACTT GGTTGGCGTG
3361 TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA ATGATTACGG CATTGATATC
3421 GTCCAACTGC ACGGAGATGA GTCGTGGCAA GAATACCAAG AGTTCCTCGG TTTGCCAGTT
3481 ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC TCAGTGCAGC TTCACAGAAA
3541 CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG GGACAGGTGA ACTTTTGGAT
3601 TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC CCGAGAGCTT ACATTTTATG
3661 TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC TTAGATTAAA TGGCGTTATT
3721 GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA AAGACTCTAA CAAAATAGCA
3781 AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT AGTATTTATT TAAGTATTGT
3841 TTGTGCACTT GCCCTAGCTT ATCGATGATA AGCTGTCAAA GATGAGAATT AATTCCACGG
3901 ACTATAGACT ATACTAGATA CTCCGTCTAC TGTACGATAC ACTTCCGCTC AGGTCCTTGT
3961 CCTTTAACGA GGCCTTACCA CTCTTTTGTT ACTCTATTGA TCCAGCTCAG CAAAGGCAGT
4021 GTGATCTAAG ATTCTATCTT CGCGATGTAG TAAAACTAGC TAGACCGAGA AAGAGACTAG
4081 AAATGCAAAA GGCACTTCTA CAATGGCTGC CATCATTATT ATCCGATGTG ACGCTGCAGC
4141 TTCTCAATGA TATTCGAATA CGCTTTGAGG AGATACAGCC TAATATCCGA CAAACTGTTT
4201 TACAGATTTA CGATCGTACT TGTTACCCAT CATTGAATTT TGAACATCCG AACCTGGGAG
4261 TTTTCCCTGA AACAGATAGT ATATTTGAAC CTGTATAATA ATATATAGTC TAGCGCTTTA
4321 CGGAAGACAA TGTATGTATT TCGGTTCCTG GAGAAACTAT TGCATCTATT GCATAGGTAA
4381 TCTTGCACGT CGCATCCCCG GTTCATTTTC TGCGTTTCCA TCTTGCACTT CAATAGCATA
4441 TCTTTGTTAA CGAAGCATCT GTGCTTCATT TTGTAGAACA AAAATGCAAC GCGAGAGCGC
4501 TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA ACAGAAATGC AACGCGAAAG
4561 CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA AAACAAAAAT GCAACGCGAC
4621 GAGAGCGCTA ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC AGAAATGCAA
4681 CGCGAGAGCG CTATTTTACC AACAAAGAAT CTATACTTCT TTTTTGTTCT ACAAAAATGC
4741 ATCCCGAGAG CGCTATTTTT CTAACAAAGC ATCTTAGATT ACTTTTTTTC TCCTTTGTGC
4801 GCTCTATAAT GCAGTCTCTT GATAACTTTT TGCACTGTAG GTCCGTTAAG GTTAGAAGAA
4861 GGCTACTTTG GTGTCTATTT TCTCTTCCAT AAAAAAAGCC TGACTCCACT TCCCGCGTTT
4921 ACTGATTACT AGCGAAGCTG CGGGTGCATT TTTTCAAGAT AAAGGCATCC CCGATTATAT
4981 TCTATACCGA TGTGGATTGC GCATACTTTG TGAACAGAAA GTGATAGCGT TGATGATTCT
5041 TCATTGGTCA GAAAATTATG AACGGTTTCT TCTATTTTGT CTCTATATAC TACGTATAGG
5101 AAATGTTTAC ATTTTCGTAT TGTTTTCGAT TCACTCTATG AATAGTTCTT ACTACAATTT
5161 TTTTGTCTAA AGAGTAATAC TAGAGATAAA CATAAAAAAT GTAGAGGTCG AGTTTAGATG
5221 CAAGTTCAAG GAGCGAAAGG TGGATGGGTA GGTTATATAG GGATATAGCA CAGAGATATA
5281 TAGCAAAGAG ATACTTTTGA GCAATGTTTG TGGAAGCGGT ATTCGCAATG GGAAGCTCCA
5341 CCCCGGTTGA TAATCAGAAA AGCCCCAAAA ACAGGAAGAT TGTATAAGCA AATATTTAAA
5401 TTGTAAACGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT
5461 TTAACGAATA GCCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG
5521 GGTTGAGTGT TGTTCCAGTT TCCAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG
5581 TCAAAGGGCG AAAAAGGGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT
5641 CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CAGTAAATCG GAAGGGTAAA CGGATGCCCC
5701 CATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA
5761 AAGGAGCGGG GGCTAGGGCG GTGGGAAGTG TAGGGGTCAC GCTGGGCGTA ACCACCACAC
5821 CCGCCGCGCT TAATGGGGCG CTACAGGGCG CGTGGGGATG ATCCACTAGT
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文献和实验相关实验
实验步骤 1. 酵母表达载体的构建 1) 用引物扩增目的基因全长片段，将它克隆到pGEM-T Easy载体上，转化大肠杆菌DH5α，分别获得阳性克隆； 2) 提取酵母表达载体pYES2和目的片段的质粒； 3) 用EcoR I和Not I双酶切pYES2载体和连有各目的片段的pGEM-T Easy载体； 4) 琼脂糖凝胶电泳分离酶切片段； 5) 回收目的
, we may find much more references regarding this issue. http://books.google.com/books?id=3mgPzNFjcWAC&pg=PA51&lpg=PA51&dq=gsk+tyrosine+phosphorylation+and+activity&source=bl&ots=x5ueTldpez&sig=CqRFJleXtEBn9OfelhODPAc28xs&hl=en&ei=KerNSu-XGcem8Abm-qmABA&sa=X&oi=book_result&ct
(119) M13F(-47) M13R(-48) pUCm-T M13F/T7 M13R pVAX1 T7 BGH pWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48) pXT7 T7 SP6 pYES2 T7 pYes2.R pLEXA pLEXA-F pLEXA-R pLNCX pLNCX-F pLNCX-R pRECEIVER CMV-F 此载体无反向引物 pSHUTTLE-CMV PSHUTTLE-CMV-F PSHUTTLE-CMV-R
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pYES3/CT载体
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