| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCas-Guide-EF1a-CD4 |
| 质粒类型: | CRISPR/Cas9载体；基因敲除载体；哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | U6 |
| 载体大小: | 11006 bp |
| 5' 测序引物及序列: | 5’ gatcgATGGGAGGTGGTATGGGAGGg 3’ |
| 3' 测序引物及序列: | 5’ aaaacCCTCCCATACCACCTCCCATc 3’ |
| 载体标签: | Myc(C-ter） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | -- |
| 产品目录号: | ZY100022 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 询价记录
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCas-Guide-EF1a-CD4
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pCas-Guide-EF1a-CD4载体特征
All-in-one vector
Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
BamH I and BsmB I sites are used for genomic target sequence cloning
Expresses gRNA containing the inserted target sequence and the gRNA scaffold
EF1a promoter driven CD4 to enrich transfected cells
CF3 primer (GE100008) can be used to sequence the target sequence after cloning
载体序列
LOCUS Exported File 11006 bp ds-DNA circular SYN 23-七月-2016
DEFINITION Mammalian vector for co-expressing a guide RNA (gRNA) together with
Cas9 and CD4, for genome editing using the S. pyogenes CRISPR/Cas9
system.
ACCESSION .
VERSION .
KEYWORDS pCas-Guide-EF1a-CD4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11006)
AUTHORS OriGene
TITLE Direct Submission
JOURNAL Exported 2016-07-23 from SnapGene Viewer 1.5.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11006
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
promoter 90..330
/note="U6"
/note="RNA polymerase III promoter for human U6 snRNA"
/note="color: #000000; direction: RIGHT"
misc_RNA 384..459
/note="gRNA scaffold"
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
/note="color: #00ccff; direction: RIGHT"
terminator 460..465
/note="polIII terminator"
/note="RNA polymerase III transcription terminator"
/note="color: #ffffff"
promoter 480..1658
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
/note="color: #000000; direction: RIGHT"
intron 711..1649
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
/note="color: #------"
CDS 1679..2950
/codon_start=1
/product="truncated human CD4 cell surface glycoprotein
lacking the cytoplasmic tail"
/note="CD4 (truncated)"
/note="This feature has 2 segments:
???1:?1679?..?1753?/?#ff6600?/?signal?peptide
???2:?1754?..?2950?/?#ff6600
Cleavage site after base 1753"
/translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
IVLGGVAGLLLFIGLGIFFCVRCRH"
enhancer 3560..3863
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
/note="color: #000000"
promoter 3864..4067
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
/note="color: #000000; direction: RIGHT"
promoter 4093..4111
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: RIGHT"
CDS 4170..8273
/codon_start=1
/locus_tag="
"
/product="Cas9 (Csn1) endonuclease from the?Streptococcus
pyogenes?Type II CRISPR/Cas system"
/note="Cas9"
/note="generates RNA-guided double strand breaks in DNA"
/note="color: #993366"
/protein_id="
"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8274..8294
/codon_start=1
/locus_tag="
"
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/note="
"
/note="color: #993366"
/protein_id="
"
/translation="PKKKRKV"
CDS 8301..8321
/codon_start=1
/locus_tag="
"
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/note="
"
/note="color: #993366"
/protein_id="
"
/translation="PKKKRKV"
CDS 8343..8372
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/note="color: #cc99b2"
/translation="EQKLISEEDL"
CDS 8391..8414
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/note="color: #cc99b2"
/translation="DYKDDDDK"
polyA_signal 8461..9083
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(9204..9792)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(9967..10827)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
???1:??9967?..?10758?/?#ccffcc
???2:?10759?..?10827?/?#ccffcc?/?signal?sequence
Cleavage site after base 10758"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 gaattcccca gtggaaagac gcgcaggcaa aacgcaccac gtgacggagc gtgaccgcgc
61 gccgagcgcg cgccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
121 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
301 tcttgggttt atatatcttg tggaaaggac gcgggatcca ctggaccagg cagcagcgtc
361 agaagacttt tttggaacgt ctcgttttag agctagaaat agcaagttaa aataaggcta
421 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggtg tacacgtgag
481 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
541 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
601 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
661 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
721 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
781 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
841 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
901 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
961 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
1021 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
1081 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
1141 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
1201 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
1261 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
1321 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
1381 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
1441 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
1501 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
1561 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
1621 tggttcaaag tttttttctt ccatttcagg tgtcgtgact atagggcggc cggacgtgat
1681 gaaccgggga gtccctttta ggcacttgct tctggtgctg caactggcgc tcctcccagc
1741 agccactcag ggaaagaaag tggtgctggg caaaaaaggg gatacagtgg aactgacctg
1801 tacagcttcc cagaagaaga gcatacaatt ccactggaaa aactccaacc agataaagat
1861 tctgggaaat cagggctcct tcttaactaa aggtccatcc aagctgaatg atcgcgctga
1921 ctcaagaaga agcctttggg accaaggaaa ctttcccctg atcatcaaga atcttaagat
1981 agaagactca gatacttaca tctgtgaagt ggaggaccag aaggaggagg tgcaattgct
2041 agtgttcgga ttgactgcca actctgacac ccacctgctt caggggcaga gcctgaccct
2101 gaccttggag agcccccctg gtagtagccc ctcagtgcaa tgtaggagtc caaggggtaa
2161 aaacatacag ggggggaaga ccctctccgt gtctcagctg gagctccagg atagtggcac
2221 ctggacatgc actgtcttgc agaaccagaa gaaggtggag ttcaaaatag acatcgtggt
2281 gctagctttc cagaaggcct ccagcatagt ctataagaaa gagggggaac aggtggagtt
2341 ctccttccca ctcgccttta cagttgaaaa gctgacgggc agtggcgagc tgtggtggca
2401 ggcggagagg gcttcctcct ccaagtcttg gatcaccttt gacctgaaga acaaggaagt
2461 gtctgtaaaa cgggttaccc aggaccctaa gctccagatg ggcaagaagc tcccgctcca
2521 cctcaccctg ccccaggcct tgcctcagta tgctggctct ggaaacctca ccctggccct
2581 tgaagcgaaa acaggaaagt tgcatcagga agtgaacctg gtggtgatga gagccactca
2641 gctccagaaa aatttgacct gtgaggtgtg gggacccacc tcccctaagc tgatgctgag
2701 cttgaaactg gagaacaagg aggcaaaggt ctcgaagcgg gagaaggcgg tgtgggtgct
2761 gaaccctgag gcggggatgt ggcagtgtct gctgagtgac tcgggacagg tcctgctgga
2821 atccaacatc aaggttctgc ccacatggtc caccccggtg cagccaatgg ccctgattgt
2881 gctggggggc gtcgccggcc tcctgctttt cattgggcta ggcatcttct tctgtgtcag
2941 gtgccggcac accggttagg atatcagatc cccgggatgc agaaattgat gatctattaa
3001 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga
3061 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg
3121 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt
3181 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
3241 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc
3301 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
3361 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
3421 tccatcagaa gctggtcgag atccggaacc cttaatataa cttcgtataa tgtatgctat
3481 acgaagttat taggtccact agttattaat agtaatcaat tacggggtca ttagttcata
3541 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
3601 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
3661 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
3721 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
3781 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
3841 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
3901 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
3961 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
4021 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
4081 gtcagaattt tgtaatacga ctcactatag ggcggccggg aattcgtcga ctggaaccgg
4141 taccgaggag atctgccgcc gcgatcgcca tggataagaa atactcaata ggactggata
4201 ttggcacaaa tagcgtcgga tgggctgtga tcactgatga atataaggtt ccttctaaaa
4261 agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc
4321 ttctgtttga cagtggagag acagccgaag ctactagact caaacggaca gctaggagaa
4381 ggtatacaag acggaagaat aggatttgtt atctccagga gattttttca aatgagatgg
4441 ccaaagtgga tgatagtttc tttcatagac ttgaagagtc ttttttggtg gaagaagaca
4501 agaagcatga aagacatcct atttttggaa atatagtgga tgaagttgct tatcacgaga
4561 aatatccaac tatctatcat ctgagaaaaa aattggtgga ttctactgat aaagccgatt
4621 tgcgcctgat ctatttggcc ctggcccaca tgattaagtt tagaggtcat tttttgattg
4681 agggcgatct gaatcctgat aatagtgatg tggacaaact gtttatccag ttggtgcaaa
4741 cctacaatca actgtttgaa gaaaacccta ttaacgcaag tggagtggat gctaaagcca
4801 ttctttctgc aagattgagt aaatcaagaa gactggaaaa tctcattgct cagctccccg
4861 gtgagaagaa aaatggcctg tttgggaatc tcattgcttt gtcattgggt ttgaccccta
4921 attttaaatc aaattttgat ttggcagaag atgctaaact ccagctttca aaagatactt
4981 acgatgatga tctggataat ctgttggctc aaattggaga tcaatatgct gatttgtttt
5041 tggcagctaa gaatctgtca gatgctattc tgctttcaga catcctgaga gtgaatactg
5101 aaataactaa ggctcccctg tcagcttcaa tgattaaacg ctacgatgaa catcatcaag
5161 acttgactct tctgaaagcc ctggttagac aacaacttcc agaaaagtat aaagaaatct
5221 tttttgatca atcaaaaaac ggatatgcag gttatattga tggcggcgca agccaagaag
5281 aattttataa atttatcaaa ccaattctgg aaaaaatgga tggtactgag gaactgttgg
5341 tgaaactgaa tagagaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc
5401 cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc
5461 catttctgaa agacaataga gagaagattg aaaaaatctt gacttttagg attccttatt
5521 atgttggtcc attggccaga ggcaatagta ggtttgcatg gatgactcgg aagtctgaag
5581 aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat
5641 ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagtg ctgccaaaac
5701 atagtttgct ttatgagtat tttaccgttt ataacgaatt gacaaaggtc aaatatgtta
5761 ctgaaggaat gagaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatc
5821 tgctcttcaa aacaaatagg aaagtgaccg ttaagcaact gaaagaagat tatttcaaaa
5881 aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcac
5941 tgggtacata ccatgatttg ctgaaaatta ttaaagataa agattttttg gataatgaag
6001 aaaatgaaga catcctggag gatattgttc tgacattgac cctgtttgaa gatagggaga
6061 tgattgagga aagacttaaa acatacgctc acctctttga tgataaggtg atgaaacagc
6121 ttaaaagacg cagatatact ggttggggaa ggttgtccag aaaattgatt aatggtatta
6181 gggataagca atctggcaaa acaatactgg attttttgaa atcagatggt tttgccaatc
6241 gcaattttat gcagctcatc catgatgata gtttgacatt taaagaagac atccaaaaag
6301 cacaagtgtc tggacaaggc gatagtctgc atgaacatat tgcaaatctg gctggtagcc
6361 ctgctattaa aaaaggtatt ctccagactg tgaaagttgt tgatgaattg gtcaaagtga
6421 tggggcggca taagccagaa aatatcgtta ttgaaatggc aagagaaaat cagacaactc
6481 aaaagggcca gaaaaattcc agagagagga tgaaaagaat cgaagaaggt atcaaagaac
6541 tgggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc
6601 tctatctcta ttatctccaa aatggaagag atatgtatgt ggaccaagaa ctggatatta
6661 ataggctgag tgattatgat gtcgatcaca ttgttccaca aagtttcctt aaagacgatt
6721 caatagacaa taaggtcctg accaggtctg ataaaaatag aggtaaatcc gataacgttc
6781 caagtgaaga agtggtcaaa aagatgaaaa actattggag acaacttctg aacgccaagc
6841 tgatcactca aaggaagttt gataatctga ccaaagctga aagaggaggt ttgagtgaac
6901 ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg
6961 tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttatta
7021 gagaggttaa agtgattacc ctgaaatcta aactggtttc tgacttcaga aaagatttcc
7081 aattctataa agtgagagag attaacaatt accatcatgc ccatgatgcc tatctgaatg
7141 ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaaagcgag tttgtctatg
7201 gtgattataa agtttatgat gttaggaaaa tgattgctaa gtctgagcaa gaaataggca
7261 aagcaaccgc aaagtatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta
7321 cacttgcaaa tggagagatt cgcaaacgcc ctctgatcga aactaatggg gaaactggag
7381 aaattgtctg ggataaaggg agagattttg ccacagtgcg caaagtgttg tccatgcccc
7441 aagtcaatat cgtcaagaaa acagaagtgc agacaggcgg attctctaag gagtcaattc
7501 tgccaaaaag aaattccgac aagctgattg ctaggaaaaa agactgggac ccaaaaaaat
7561 atggtggttt tgatagtcca accgtggctt attcagtcct ggtggttgct aaggtggaaa
7621 aagggaaatc caagaagctg aaatccgtta aagagctgct ggggatcaca attatggaaa
7681 gaagttcctt tgaaaaaaat cccattgact ttctggaagc taaaggatat aaggaagtta
7741 aaaaagacct gatcattaaa ctgcctaaat atagtctttt tgagctggaa aacggtagga
7801 aacggatgct ggctagtgcc ggagaactgc aaaaaggaaa tgagctggct ctgccaagca
7861 aatatgtgaa ttttctgtat ctggctagtc attatgaaaa gttgaagggt agtccagaag
7921 ataacgaaca aaaacaattg tttgtggagc agcataagca ttatctggat gagattattg
7981 agcaaatcag tgaattttct aagagagtta ttctggcaga tgccaatctg gataaagttc
8041 ttagtgcata taacaaacat agagacaaac caataagaga acaagcagaa aatatcattc
8101 atctgtttac cttgaccaat cttggagcac ccgctgcttt taaatacttt gatacaacaa
8161 ttgataggaa aagatatacc tctacaaaag aagttctgga tgccactctt atccatcaat
8221 ccatcactgg tctttatgaa acacgcattg atttgagtca gctgggaggt gaccccaaga
8281 aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt ggacacgcgt acgcggccgc
8341 tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg
8401 atgacgacga taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc
8461 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
8521 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
8581 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
8641 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
8701 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
8761 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagaggcgg
8821 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
8881 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
8941 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
9001 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
9061 actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat cagctcactc
9121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtccgt
9181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
9241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
9301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
9361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
9421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
9481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
9541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
9601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
9661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
9721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
9781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac
9841 gcgtaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
9901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
9961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
10021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
10081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
10141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
10201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
10261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
10321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
10381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
10441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
10501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
10561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
10621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
10681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
10741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
10801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
10861 tcagggttat tgtctcatga tgatattatt ttatcttgtg caatgtaaca tcagagattt
10921 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
10981 gggatatcag ctggatggca gttaac
//
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pCas-Guide-EF1a-CD4载体
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