YCplac22载体

LOCUS       YCplac22	4854 bp 	DNA	SYN
DEFINITION  YCplac22
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="YCplac22"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     misc_feature    504..1356
                     /label="CEN4"
     gene            complement(2066..2740)
                     /label="TRP1"
                     /gene="TRP1"
     CDS             complement(2066..2740)
                     /label="ORF frame 3"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     promoter        2983..3011
                     /label="AmpR_promoter"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     gene            3053..3913
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     CDS             3053..3913
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4068..4687
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
  541 tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat
  601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
  661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
  721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
  781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
  841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
  901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
  961 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa
 1021 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
 1081 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
 1141 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
 1201 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
 1261 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
 1321 acagttttac agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga
 1381 gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
 1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
 1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
 1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
 1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
 1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
 1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
 1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
 1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
 1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
 1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
 2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
 2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
 2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
 2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
 2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
 2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
 2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa
 2461 tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa
 2521 ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc
 2581 ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt
 2641 cagcatcgga atctagtgca cattctgcgg cctctgtgct ctgcaagccg caaactttca
 2701 ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt
 2761 gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc
 2821 cttttctttt ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt
 2881 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
 2941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
 3001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
 3061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
 3121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
 3181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
 3241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
 3301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
 3361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
 3421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
 3481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
 3541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
 3601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
 3661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
 3721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
 3781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
 3841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
 3901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
 3961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
 4021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
 4081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
 4141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
 4201 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
 4261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
 4321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
 4381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
 4441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
 4501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
 4561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
 4621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
 4681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
 4741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
 4801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga