YCplac33载体

LOCUS       YCplac33	5603 bp 	DNA	SYN
DEFINITION  YCplac33
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="YCplac33"
                     /mol_type="other DNA"
     promoter        142..171
                     /label="lac_promoter"
     misc_feature    185..207
                     /label="M13_pUC_rev_primer"
     promoter        206..224
                     /label="M13_reverse_primer"
     misc_feature    287..442
                     /label="lacZ_a"
     promoter        complement(290..306)
                     /label="M13_forward20_primer"
     misc_feature    complement(299..321)
                     /label="M13_pUC_fwd_primer"
     misc_feature    504..1356
                     /label="CEN4"
     CDS             complement(2562..3365)
                     /label="ORF frame 1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     gene            complement(2565..3365)
                     /label="URA3"
                     /gene="URA3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             2854..3330
                     /label="ORF frame 1"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        3732..3760
                     /label="AmpR_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     gene            3802..4662
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             3802..4662
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4817..5436
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
   61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
  121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
  181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
  241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
  301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
  361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
  421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
  481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
  541 tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat
  601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
  661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
  721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
  781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
  841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
  901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
  961 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa
 1021 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
 1081 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
 1141 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
 1201 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
 1261 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
 1321 acagttttac agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga
 1381 gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
 1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
 1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
 1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
 1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
 1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
 1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
 1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
 1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
 1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
 1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
 2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
 2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
 2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
 2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
 2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
 2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
 2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatcgatg
 2461 ataagctgtc aaacatgaga attgggtaat aactgatata attaaattga agctctaatt
 2521 tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg ctggccgcat
 2581 cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac cttagcatcc
 2641 cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt agagaccaca
 2701 tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa acccacaccg
 2761 ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct ttgagcaata
 2821 aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt agtatattct
 2881 ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc
 2941 tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg
 3001 tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga gtactgcaat
 3061 ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa attgtacttg
 3121 gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt caagatatcc
 3181 acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc cagtaattcc
 3241 ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg catgatatta
 3301 aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata tgtagctttc
 3361 gacatgattt atcttcgttt cctgcatgtt tttgttctgt gcagttgggt taagaatact
 3421 gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct aagtctgtgc
 3481 tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaagaaac
 3541 cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc taattcttga
 3601 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
 3661 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
 3721 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
 3781 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
 3841 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
 3901 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
 3961 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
 4021 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
 4081 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
 4141 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
 4201 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
 4261 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
 4321 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
 4381 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
 4441 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
 4501 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
 4561 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
 4621 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
 4681 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
 4741 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
 4801 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
 4861 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
 4921 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
 4981 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
 5041 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
 5101 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
 5161 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
 5221 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
 5281 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
 5341 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
 5401 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
 5461 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
 5521 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
 5581 tcagtgagcg aggaagcgga aga