pYC16载体

pYC16载体

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  • ZYbscience
  • 中国/美国
  • ZY63913
  • 2025年07月14日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pYC16

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品人: ZYbscience
    载体名称: pYC16
    质粒类型: 酿酒酵母载体；过表达载体；Recipient载体
    高拷贝/低拷贝: 高拷贝
    克隆方法: 限制性内切酶，多克隆位点
    启动子: TEF1
    载体大小: 6418 bp
    5' 测序引物及序列: KS primer 5’TCGAGGTCGACGGTATC 3’
    3' 测序引物及序列: GFP-R primer 5'TAGTTTTCCAGTAGTGCAAATAAATTTAA3'
    载体标签: GFP
    载体抗性: 氨苄青霉素
    筛选标记: URA3
    克隆菌株: DH10B感受态细胞 ,30°C
    宿主细胞（系）: 出芽酵母（酿酒酵母）、白色念珠菌
    备注: --
    产品目录号: ZY63913
    稳定性: 瞬表达
    组成型/诱导型: 组成型
    病毒/非病毒: 非病毒

    载体质粒图谱和多克隆位点信息

    pYC16载体图谱



    pYC16多克隆位点

    载体简介

    载体序列

    LOCUS       pYC16	6418 bp 	DNA	SYN
DEFINITION  pYC16
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="pYC16"
                     /mol_type="other DNA"
     promoter        668..696
                     /label="AmpR_promoter"
     gene            738..1598
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             738..1598
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      1753..2372
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2724..2746
                     /label="M13_pUC_rev_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2745..2763
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2780..2799
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3218..3234
                     /label="pBluescriptSK_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             3238..3954
                     /label="ORF frame 1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     gene            3244..3951
                     /label="GFP (variant)"
                     /gene="GFP (variant)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3268..3296)
                     /label="GFP_R_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3312..3331)
                     /label="GFPfusionREV_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     misc_feature    3418..3447
                     /label="Y66W (ECFP)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     misc_feature    3891..3911
                     /label="GFP_F_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3994..4010)
                     /label="pBluescriptKS_primer"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK*"
     CDS             4246..4830
                     /label="ORF frame 1"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(4424..4442)
                     /label="T7_promoter"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(4452..4468)
                     /label="M13_forward20_primer"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(4461..4483)
                     /label="M13_pUC_fwd_primer"
                     /translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
                     RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
                     EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
                     LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             complement(5199..6002)
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     gene            complement(5202..6002)
                     /label="URA3"
                     /gene="URA3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             5491..5967
                     /label="ORF frame 1"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
ORIGIN
    1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
   61 cttaGCAGGT TAGACTAACA TGCAAAAAGT TTATATTCAT TTTTGTAGTT ATTCTTGTTT
  121 TCATTAGGAT GATTACACAC TGTGGATGTG GATTAATCGA TCATTGTTAA TTTAGTGTAG
  181 CTACTACCAT TTCAAACAAA ACGTAAATTC TTGCAGTCGT ACTGGATCTG TGAATCTATT
  241 AGTATATATG AATTAAAGTA GCTTGTACAT ATTATTCTGT TGAATCATAT CGCAGAGCAT
  301 TGGTTGAAAT CCAAAATATA AAAATGTAAT ATCACAAAAA ATAATACTAA TTCTAACATT
  361 AATGGGTCAG ATTTTTAGTG AATACTTAAA TTTATAATAT CGTCTATTTA AGCTAGCAAA
  421 TGGAACAACA TTTAAAGTAA GAACATCATA TCTACATGAA AATGTATATT TCAATCTGAC
  481 TAATAACGCA GAGCACATCT TTCAGTGATG TCTGTCACAT GATCAAAAAG AATTGTATTT
  541 AAATAATTCA TAATAAAAGC TTAAAAAATT Acaataatga aaataaagtA AATAATGACA
  601 TGGGTAAGAG TTCCGAAATA GAATCTTAGT GTACAAAACA AAAATTGCAT CATTAGAGAT
  661 CCCCacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
  721 attgaaaaag gaagagtATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
  781 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
  841 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
  901 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
  961 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
 1021 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
 1081 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
 1141 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
 1201 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
 1261 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
 1321 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
 1381 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
 1441 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
 1501 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
 1561 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAAct gtcagaccaa gtttactcat
 1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
 1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
 1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
 1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
 1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
 1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
 1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
 2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
 2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
 2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
 2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
 2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
 2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
 2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
 2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
 2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
 2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
 2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
 2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
 2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gAGCTCATAG
 2821 CTTCAAAATG TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC
 2881 CGTACCACTT CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG
 2941 GTGTCGTTAA TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT
 3001 TTTCTTCGTC GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT
 3061 TTTTTGATTT TTTTCTCTTT CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT
 3121 CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC
 3181 TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGTTTTCT AGaactagtg gatcctaatg
 3241 AGTAAAGGAG AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT
 3301 GTTAATGGGC ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA
 3361 CTTACCCTTA AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGCC AACACTTGTC
 3421 ACTACTTTGA CTTGGGGTGT TCAATGCTTT GCTAGATACC CAGATCATAT GAAACAGCAT
 3481 GACTTTTTCA AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA
 3541 GATGACGGGA ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT
 3601 AGAATCGAGT TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG
 3661 GAATACAACG CTATTTCAGA TAATGTATAC ATCACTGCAG ACAAACAAAA GAATGGAATC
 3721 AAAGCTAATT TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT
 3781 TATCAACAAA ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG
 3841 TCCACACAAT CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT
 3901 GAGTTTGTAA CAGCTGCTGG GATTACACTA GGCATGGATG AACTATACAA Atagagatcc
 3961 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacCTCGA GAACACAACC
 4021 CACAGCTACC ACCATCAACA ATATTTATAT ATATAACGTA CACATAGAAA TCACACAAAC
 4081 AGAGTATTTA TTCTTAACTA CATGAACTAC CATCAGACCG TCTGGGCCAC TATATAATGT
 4141 GCCATTCATA AACGTGATCA CTTTACGTAG CAGGCAACCC CAGGTGAAAA TTTTTCAGCG
 4201 AGCTGCCAGA TTGTCAGGTG AAAAACTGAA AAAAACTTCT GGGCGATGAG CTTGTGGCGG
 4261 GAAATTAAGT ATATAAAGCA GTTAGTTTCT TCTGCTTCTT GTGGTTCTGG GTATTCTTGT
 4321 AATATTTGGA AGAATAGAAA ATCAAGACTA CTGCCTTTCT TTTCATATTA TAGAGGAAGA
 4381 AATACGCACG AACACGATAT AGAGGTAAAG GTACCcaatt cgccctatag tgagtcgtat
 4441 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
 4501 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
 4561 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc
 4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
 4681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
 4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
 4801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
 4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
 4921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
 4981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
 5041 tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt
 5101 gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc tctaatttgt
 5161 gagtttagta tacatgcatt tacttataat acagttttTT AGTTTTGCTG GCCGCATCTT
 5221 CTCAAATATG CTTCCCAGCC TGCTTTTCTG TAACGTTCAC CCTCTACCTT AGCATCCCTT
 5281 CCCTTTGCAA ATAGTCCTCT TCCAACAATA ATAATGTCAG ATCCTGTAGA GACCACATCA
 5341 TCCACGGTTC TATACTGTTG ACCCAATGCG TCTCCCTTGT CATCTAAACC CACACCGGGT
 5401 GTCATAATCA ACCAATCGTA ACCTTCATCT CTTCCACCCA TGTCTCTTTG AGCAATAAAG
 5461 CCGATAACAA AATCTTTGTC GCTCTTCGCA ATGTCAACAG TACCCTTAGT ATATTCTCCA
 5521 GTAGATAGGG AGCCCTTGCA TGACAATTCT GCTAACATCA AAAGGCCTCT AGGTTCCTTT
 5581 GTTACTTCTT CTGCCGCCTG CTTCAAACCG CTAACAATAC CTGGGCCCAC CACACCGTGT
 5641 GCATTCGTAA TGTCTGCCCA TTCTGCTATT CTGTATACAC CCGCAGAGTA CTGCAATTTG
 5701 ACTGTATTAC CAATGTCAGC AAATTTTCTG TCTTCGAAGA GTAAAAAATT GTACTTGGCG
 5761 GATAATGCCT TTAGCGGCTT AACTGTGCCC TCCATGGAAA AATCAGTCAA GATATCCACA
 5821 TGTGTTTTTA GTAAACAAAT TTTGGGACCT AATGCTTCAA CTAACTCCAG TAATTCCTTG
 5881 GTGGTACGAA CATCCAATGA AGCACACAAG TTTGTTTGCT TTTCGTGCAT GATATTAAAT
 5941 AGCTTGGCAG CAACAGGACT AGGATGAGTA GCAGCACGTT CCTTATATGT AGCTTTCGAC
 6001 ATgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa gaatactggg
 6061 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
 6121 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga
 6181 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctgt ggtatggtgc
 6241 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
 6301 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
 6361 accgtctccg ggagGtgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga

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