| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pcDNA3.1/Myc-His C , pcDNA3.1/Myc-HisC |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5490 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | C-His, C-Myc |
| 载体抗性: | Ampicillin 氨苄 |
| 筛选标记: | Neomycin (G418) |
| 备注: |
|
| 产品目录号: | ZY855-20 |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
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- ZYbscience
- 中国/美国
- ZY855-20
- 2025年07月13日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pcDNA3.1/Myc-His C
- 库存：
20
- 供应商：
泽叶生物
pcDNA3.1/Myc-HisC载体基本信息
pcDNA3.1/Myc-HisC载体质粒图谱和多克隆位点信息
pcDNA3.1/Myc-HisC载体简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
pcDNA3.1/Myc-HisC载体序列
LOCUS pcDNA3.1(+)/myc-HisC 5490 bp DNA circular SYN 2-SEP-2003 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..5490 /invitrogen="128" /vntifkey="98" CDS 1038..1055 /invitrogen="1010000" /vntifkey="4" /label=6xHis CDS complement(4494..5354) /invitrogen="1020000" /vntifkey="4" /label=Amp(R) polyA_site 1084..1308 /invitrogen="1870000" /vntifkey="26" /label=BGH\pA primer complement(1078..1095) /invitrogen="1910000" /vntifkey="27" /label=BGH\reverse\primer promoter complement(5355..5453) /invitrogen="2330000" /vntifkey="30" /label=bla\promoter primer 769..789 /invitrogen="1930000" /vntifkey="27" /label=CMV\forward\primer promoter 232..819 /invitrogen="2180000" /vntifkey="29" /label=CMV\promoter CDS 993..1022 /invitrogen="1090000" /vntifkey="4" /label=c-myc\epitope rep_origin 1354..1782 /invitrogen="2500000" /vntifkey="33" /label=f1\origin misc_feature 902..995 /invitrogen="1470000" /vntifkey="21" /label=MCS CDS 2193..2987 /invitrogen="1260000" /vntifkey="4" /label=Neo(R) rep_origin complement(3676..4349) /invitrogen="2540000" /vntifkey="33" /label=pUC\origin promoter 1787..2157 /invitrogen="2290000" /vntifkey="29" /label=SV40\early\promoter polyA_signal 3163..3293 /invitrogen="1840000" /vntifkey="25" /label=SV40\pA primer 863..882 /invitrogen="2100000" /vntifkey="27" /label=T7\primer promoter 863..879 /invitrogen="2370000" /vntifkey="30" /label=T7\promoter BASE COUNT 1273 a 1427 c 1397 g 1393 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 961 gcacagtggc ggccgctcga ggtcacccat tcgaacaaaa actcatctca gaagaggatc 1021 tgaatatgca taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct 1081 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1141 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1201 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1261 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 1321 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 1381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 1441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 1501 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 1561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 1621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt 1741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 1801 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc 1861 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 1921 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 1981 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 2041 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 2101 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 2161 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 2221 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 2281 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 2341 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 2401 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 2461 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 2521 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 2581 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 2641 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 2701 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 2761 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 2821 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 2881 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 2941 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 3001 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 3061 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 3121 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 3181 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 3241 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 3301 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 3421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 3481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 3541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 3601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 3661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3901 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 4141 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 4381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 4441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 4501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 4561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 4621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 4681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 4741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 4801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 4861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 4921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 4981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 5041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 5101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 5161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 5221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 5281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 5341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 5401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 5461 acatttcccc gaaaagtgcc acctgacgtc
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pcDNA3.1/Myc-His C载体
¥1350
