| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCMV6-XL4 |
| 质粒类型: | 哺乳动物表达载体；克隆载体；体外转录载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4704 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | hGH pA rev 5’CCAGCTTGGTTCCCAATAGA 3’ |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
- ¥1500
- ZYbscience
- 中国/美国
- ZY
- 2025年07月15日
10 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCMV6-XL4
- 库存：
20
- 供应商：
泽叶生物
pCMV6 XL4载体基本信息
pCMV6 XL4载体质粒图谱和多克隆位点信息
pCMV6 XL4载体简介
pCMV6-XL4载体
Selection marker in E. coli: Ampicillin-resistance
Selection marker in mammalian cells: None. For transient transfection only
Promoter for in vivo expression in mammalian cells: CMV promoter
Promoter for in vitro cell free system: T7 (for pCMV6-XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)
Cloning sites: EcoRI and SalI. While EcoRI is still preserved, SalI is destroyed upon cloning.
Restriction sites for removing insert: NotI. *Two NotI sites are flanking the cloning sites in the vector.
Cell line suitable for transfection: COS, 293, Hela, CHO, NIH3T3, Mouse L cell, etc.
Transcription termination and polyadenylation signals: from human growth hormone (hGH) gene.
pCMV6 XL4载体序列
LOCUS pCMV6-XL4 4707 bp DNA circular SYN 01-JUL-1998
DEFINITION Cloning vector pCMV6-XL4, complete sequence.
ACCESSION AF067196
VERSION AF067196.1 GI:3273767
KEYWORDS .
SOURCE Cloning vector PCMV6-XL4
ORGANISM Cloning vector PCMV6-XL4
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4707
/organism="Cloning vector PCMV6-XL4"
/mol_type="genomic DNA"
/host="Escherichia coli"
/db_xref="taxon:77618"
promoter 201..926
/note="CMV"
promoter 953..971
/note="T7 promoter"
misc_feature 972..1030
/note="multiple cloning site"
primer_bind 1031..1048
/note="M13 reverse primer"
polyA_signal 1063..1684
/note="human growth hormone"
rep_origin 1740..2047
/note="SV40 origin"
rep_origin 2457..3129
/note="ColE1"
CDS complement(3274..4134)
/codon_start=1
/transl_table=11
/product="ampicillin resistance protein"
/protein_id="AAC24837.1"
/db_xref="GI:3273768"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 4267..4679
/note="f1"
ORIGIN
1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
961 tcactatagg gcggccgcga attcagatct ggtaccgata tcaagcttgt cgactctaga
1021 ttgcggccgc ggtcatagct gtttcctgaa catgtgatcc cgggtggcat ccctgtgacc
1081 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc
1141 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt
1201 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg
1261 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct
1321 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca
1381 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca
1441 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg
1501 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc
1561 agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt
1621 tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg
1681 aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc
1741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
1801 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
1861 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
1921 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
1981 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
2041 aaaagctcct cgaggaactg aaaaaccaga aagttaattc cctatagtga gtcgtattaa
2101 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
2161 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
2221 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
2281 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
2341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
2401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
2461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
2521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
2581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
2701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
2941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
3121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
3181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
3241 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
3301 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
3361 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
3421 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
3481 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
3541 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
3601 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
3661 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
3721 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
3781 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
3841 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
3901 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
3961 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
4021 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
4081 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
4141 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
4201 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
4261 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4321 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
4381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
4501 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4561 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
4621 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
4681 atttaacgcg aattttaaca aaatatt
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
pCMV6-XL4载体
¥1500
