| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pKD46 |
| 质粒类型: | 细菌表达；基因敲除系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6329 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | -- |
| 筛选标记: | -- |
| 备注: | pKD46 is more efficient than pKD20 for making gene disruptions. |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
- ¥1500
- ZYbscience
- 中国/美国
- ZY
- 2025年07月11日
11 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pKD46
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pKD46 6329 bp DNA circular SYN 11-SEP-2001
DEFINITION Red recombinase plasmid pKD46, complete sequence.
ACCESSION AY048746
VERSION AY048746.1 GI:15554345
KEYWORDS .
SOURCE Red recombinase plasmid pKD46
ORGANISM Red recombinase plasmid pKD46
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6329)
AUTHORS Datsenko,K.A. and Wanner,B.L.
TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12
using PCR products
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000)
PUBMED 10829079
REFERENCE 2 (bases 1 to 6329)
AUTHORS Datsenko,K.A. and Wanner,B.L.
TITLE Direct Submission
JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University,
Lilly Hall of Life Sciences, West Lafayette, IN 47907, USA
FEATURES Location/Qualifiers
source 1..6329
/organism="Red recombinase plasmid pKD46"
/mol_type="genomic DNA"
/db_xref="taxon:170495"
CDS complement(11..889)
/codon_start=1
/transl_table=11
/product="araC protein"
/protein_id="AAL02045.1"
/db_xref="GI:15554346"
misc_feature complement(1040..1068)
/note="Pc"
misc_feature 1165..1192
/note="ParaB"
CDS 1244..1660
/note="recombination;138"
/codon_start=1
/transl_table=11
/product="gam protein"
/protein_id="AAL02046.1"
/db_xref="GI:15554347"
CDS 1666..2451
/note="recombination;261"
/codon_start=1
/transl_table=11
/product="bet protein"
/protein_id="AAL02047.1"
/db_xref="GI:15554348"
CDS 2448..3128
/note="exo"
/codon_start=1
/transl_table=11
/product="exonuclease"
/protein_id="AAL02048.1"
/db_xref="GI:15554349"
misc_feature 3118..3387
/note="tL3"
CDS 3125..3307
/note="60a"
/codon_start=1
/transl_table=11
/product="orf60a protein"
/protein_id="AAL02051.1"
/db_xref="GI:15554352"
CDS complement(3496..4446)
/codon_start=1
/transl_table=11
/product="repA101 protein"
/protein_id="AAL02049.1"
/db_xref="GI:15554350"
rep_origin 4450..5190
/note="oriR101"
CDS complement(5338..6198)
/note="bla; ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAL02050.1"
/db_xref="GI:15554351"
ORIGIN
1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga
1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc
1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat
1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga
1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc
1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt
1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac
1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc
1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa
1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc
1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc
1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc
1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca
1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa
2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg
2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga
2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact
2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt
2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc
2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa
2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca
2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc
2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc
2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg
2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg
2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga
2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg
2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg
2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga
2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc
3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg
3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat
3121 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt
3181 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca
3241 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt
3301 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg
3361 ccaataccag tagaaacaga cgaagaatcc atgggtatgg acagttttcc ctttgatatg
3421 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
3481 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
3541 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
3601 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
3661 ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
3721 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
3781 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
3841 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
3901 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
3961 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
4021 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
4081 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
4141 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
4201 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
4261 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
4321 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
4381 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
4441 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
4501 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
4561 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
4621 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
4681 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
4741 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
4801 aggcttaagt agcaccctcg caagctcggt tgcggccgca atcgggcaaa tcgctgaata
4861 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
4921 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
4981 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
5041 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
5101 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
5161 tgcacccagt aaggcagcgg tatcatcaac ggggtctgac gctcagtgga acgaaaactc
5221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
5341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
5401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
5461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
5521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
5581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
5641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
5701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
5761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
5821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
5881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
5941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
6001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
6061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
6121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
6181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6301 gcgcacattt ccccgaaaag tgccacctg
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
【求助】质粒有两个复制起始点，怎么复制呢？（red重组，pKD46质粒）
mnidr 我现在在做基因敲除，用的是red重组系统，pKD46质粒上携带有敲除所需的蛋白。pKD46质粒上有两个复制起始点Origin of Replication：repA101ts & oriR101 ；repA101ts是温度敏感型复制起始点。 有几个问题请教： 1.大家有用过这个pKD46的吗？oriR101是温度敏感型复制子还是条件诱导型复制子啊，我在网上查到它是温度敏感型复制起始点，我很不理解，为什么已经有一个温度
Recombineering/Lambda red-mediated gene replacement
for details, or update this list --smd 20:20, 5 March 2007 (EST) plasmids pKD46 pKD3 (chloramphenicol) pKD4 (kanamycin) pCP20 (optional) reagents L-arabinose equipment
Transposon-mediated Mutagenesis
solution, 10min 70°C Purify by passing through G50 column Step 3 � Transformation MG1655 cells harboring the pKD46 plasmid (Datsenko and Wanner, 2000, PNAS 97(12):6640-5) are induced with arabinose to activate expression of the lRed genes
技术资料暂无技术资料 索取技术资料
pKD46载体
¥1500
