Mouse IgG2b Antibody Pre-adsorbed 抗体,orb347366,Biorbyt产品图

Mouse IgG2b Antibody Pre-adsor

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  • ¥5031
  • Biorbyt已认证
  • orb347366
  • 英国
  • 2026年08月05日
  • DOT, ELISA, IHC, WB
  • Goat
  • Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      Mouse IgG2b Antibody Pre-adsorbed 抗体

    • 抗体英文名:

      Mouse IgG2b Antibody Pre-adsorbed

    • 浓度:

      1.0 mg/mL

    • 应用范围:

      DOT, ELISA, IHC, WB

    • 宿主:

      Goat

    • 适应物种:

      Mouse

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb347366

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Liquid (sterile filtered)

    • 亚型:

      IgG

    • 免疫原:

      详询

    • 规格:

      1 mg

    类别: Antibodies

    产品子类型: Secondary Antibody

    描述: Mouse IgG2b antibody

    别名: goat anti-MOUSE IgG2b Antibody, goat anti-Mouse IgG2b (gamma 2b) Antibody

    应用稀释比例: ELISA: 1:40,000 - 1:200,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000

    应用说明: Mouse IgG2b secondary antibody has been tested by dot blot and ELISA and is available in a variety of formats. Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays.

    免疫原: Mouse IgG2b heavy chain

    防腐剂: Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    纯度: Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2b coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2a and IgG3 reactivity was less than 5% against target (IgG2b, IgG). Cross-reactivity against Bovine is assessed only by IEP.

    保存条件: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

    备注: For research use only.

    产品细节图片1
    ELISA Results of Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG2b mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n orb343778). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Donkey Anti-Goat IgG HRP conjugated (p/n orb347032), and TMB substrate (p/n orb348651).

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    图标文献和实验
    相关实验
    • ANTIBODY BINDING TO PROTEIN A AND PROTEIN G

      2a ++++ ++++ Mouse IgG2b +++ +++ Mouse IgG3 ++ +++ Rat IgG1 - + Rat IgG2a - ++++ Rat IgG2b - ++ Rat IgG

    • Monoclonal Antibody Production

      Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer

    • Monoclonal Antibody Production

        Monoclonal Antibody Production     Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform

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