- ￥3276
- Biorbyt
- orb179876
- 英国
- 2026年07月22日
- FC, ICC, WB
- Human, Mouse
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- 文献和实验
- 技术资料
- 抗体名：
CD3 zeta (pY72) Antibody 抗体
- 抗体英文名：
CD3 zeta (pY72) Antibody
- 靶点：
CD3 zeta (pY72)
- 浓度：
1 mg/ml
- 应用范围：
FC, ICC, WB
- 适应物种：
Human, Mouse
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb179876
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG2b
- 免疫原：
详询
- 规格：
0.1 mg
产品子类型: Primary Antibody
描述: Mouse monoclonal antibody to CD3 zeta
克隆号: EM-26
别名: CD3 zeta chain, CD247, T3Z, TCRz, IMD25
应用说明: Western blotting: Recommended dilution: 2 - 5 μg/ml; positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended. Flow cytometry: Intracellular staining; recommended dilution: 1-9 μg/ml; positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.
免疫原: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
防腐剂: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方法: Purified by protein-A affinity chromatography.
特异性: The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
研究领域: Immunology & Inflammation
UniProt ID: P20963
Entrez 基因 ID: 919
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
. As expected, with CD3/CD28 activation, CD8+ cells that coexpressed CD5 (a human T lymphocyte antigen) proliferated, while CD5- CD8+ (NK cells) remained in G0 . After 72 h of costimulation, only 9% of CD5+ CD8+ T cells remained in G0 ; ~15% were in G1a , 21
CD5 分子 CD5 常用单克隆抗体或代号：T1，UCHT2，T101，Leul 主要表达细胞：T，Thy，BsubC T细胞 分子质量(kDa)和结构：gp67(清除剂受体) 功 能：与CD72结合，T细胞信号转导和增殖，CD5+ B细胞与自身免疫有关 CD5 Belongs to ancient scavenger receptor family
Intracellular Immunofluorescent Staining for Flow Cytometry
type and the experimental conditions. For example, to stimulate T cells to produce IFN-γ, TNF-α, IL-2, and IL-4, a combination of PMA (a phorbol ester / PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies can be used. To induce IL
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