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- 文献和实验
- 技术资料
- 抗体名:
Armenian Hamster IgG isotype Control Antibody 抗体
- 抗体英文名:
Armenian Hamster IgG isotype Control Antibody
- 浓度:
1.0 mg/mL
- 应用范围:
FC, SDS-PAGE
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb346446
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 保存条件:
详询
- 形态:
Lyophilized
- 免疫原:
详询
- 规格:
1 mg
描述: Armenian Hamster IgG isotype Control Antibody
别名: Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein
应用稀释比例: FC: 1:1000-1:5000
应用说明: ARMENIAN HAMSTER IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.
生物来源: Armenian Hamster
防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG. Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.
来源: Armenian Hamster
研究领域: Protein Biochemistry
保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.


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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
to ensure your second step reagent will recognize the primary antibody. For instance, if your primary antibody is mouse IgG2a andyour second step reagent is anti-mouse IgG1, then it will not work. Also some primary antibodies are made in hamster or rat
Rapid Isotyping of Mouse Monoclonal Antibodies
, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1 Isotype-Dependent Properties of Monoclonal
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