Armenian Hamster IgG isotype Control Antibody 抗体,orb346446,Biorbyt产品图

Armenian Hamster IgG isotype C

ontrol Antibody 抗体,orb346446,Biorbyt
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  • ¥4212
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  • orb346446
  • 英国
  • 2026年08月05日
  • FC, SDS-PAGE
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Armenian Hamster IgG isotype Control Antibody 抗体

    • 抗体英文名

      Armenian Hamster IgG isotype Control Antibody

    • 浓度

      1.0 mg/mL

    • 应用范围

      FC, SDS-PAGE

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb346446

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 保存条件

      详询

    • 形态

      Lyophilized

    • 免疫原

      详询

    • 规格

      1 mg

    类别: Antibodies

    描述: Armenian Hamster IgG isotype Control Antibody

    别名: Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein

    应用稀释比例: FC: 1:1000-1:5000

    应用说明: ARMENIAN HAMSTER IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.

    生物来源: Armenian Hamster

    防腐剂: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    纯度: ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG. Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.

    来源: Armenian Hamster

    研究领域: Protein Biochemistry

    保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    备注: For research use only.

    产品细节图片1
    Anti-CD154–facilitated alloengraftment is multilineage. Twenty mice from 2 representative experiments were phenotyped at 120 days after BMT for donor-host origin of CD4+ and CD8 + T cells, CD19 + B cells, and MAC-1 + myeloid cells. On the x-axis are shown the host and donor proportions of each of the lineages. ▪ indicates the proportion of each lineage of host origin; ▥, the proportion of each lineage that is of donor origin. On the y-axis is shown the percentage of PBLs of each lineage. Irrelevant hIgG–treated mice had no detectable donor chimerism and thus are composed entirely of host-type cells. Note that most CD4+ T cells, CD19 + B cells, and MAC-1 + myeloid cells in anti-CD154–treated mice are of donor origin. In contrast, most of the CD8 + T cells are of host origin. irrelevant hamster IgG (hIgG) (p/n orb346446).

    产品细节图片2
    SDS PAGE Results of Armenian Hamster IgG Whole Molecule Isotype Control. Lane 1: Armenian Hamster IgG Reduced [5.0 µg]. Lane 2: Armenian Hamster IgG Reduced [1.0 µg]. Lane 3: Lane 4: Armenian Hamster IgG Non-Reduced [1.0 µg]. Lane 5: Armenian Hamster IgG Non-Reduced [5.0 µg]. 4-20% Gel, Coomassie Stained.

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    图标文献和实验
    相关实验
    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    • immunofluorescence staining

      to ensure your second step reagent will recognize the primary antibody. For instance, if your primary antibody is mouse IgG2a andyour second step reagent is anti-mouse IgG1, then it will not work. Also some primary antibodies are made in hamster or rat

    • Rapid Isotyping of Mouse Monoclonal Antibodies

      , isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1  Isotype-Dependent Properties of Monoclonal

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