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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
6 months from date of receipt.
- 英文名:
E.coli WRKY2 protein
- 库存:
99
- 供应商:
Biorbyt
- 规格:
20 ug/100 ug/1 mg
| 规格: | 20 ug | 产品价格: | ¥5265.0 |
|---|---|---|---|
| 规格: | 100 ug | 产品价格: | ¥9360.0 |
| 规格: | 1 mg | 产品价格: | ¥30537.0 |
描述: This E.coli WRKY2 protein spans the amino acid sequence from region 175-405aa. Purity: Greater than 90% as determined by SDS-PAGE.
别名: WRKY2
形态: Liquid or Lyophilized powder
应用说明: This is His-tag protein
生物来源: Arabidopsis thaliana (Mouse-ear cress)
表达区域: 175-405aa
分子量: 29.8 kDa
防腐剂: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
蛋白长度: Partial
蛋白序列: FHPVSRSSSSFFQGTTEMMSVDYGNYNNRSSSHQSAEEVKPGSENIESSNLYGIETDNQNGQNKTSDVTTNTSLETVDHQEEEEEQRRGDSMAGGAPAEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKVERSREGHITEIIYKGAHNHLKPPPNRRSGMQVDGTEQVEQQQQQRDSAATWVSCNNTQQQGGSNENNVEEGSTRFEYGNQSGSIQAQTGGQYESGD
纯度: Greater than 90% as determined by SDS-PAGE.
来源: E.coli
标签: N-terminal 6xHis-tagged
研究领域: Infectious Disease & Virology
UniProt ID: Q9FG77
保存条件: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
备注: For research use only.

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文献和实验Expression of a cDNA in E.coli in order to prepare an antiserum
an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase ( LacZ
Analysis of total E.coli protein by SDS PAGE
Hahn Lab 1.In microfuge tubes,spin down 0.1 ml of uninduced cells grown to near saturation or 0.15 ml of IPTG induced cells.Remove YT (or LB)media with a pipetman or drawn out pasteur pipette and freeze cell pellets for later analysis. 2.Add 120
Expression of a cDNA in E.coli in order to prepare an antise
and prepare an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase (LacZ ), histidine tags
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