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- 2026年08月13日
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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-20°C for 1 months and -70℃ for 3 months.
- 保存条件：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days; <br>-20°C for 1 months and -70℃ for 3 months.
- 英文名：
E Protein
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/50ug/500ug
| 规格： | 1mg | 产品价格： | ¥7925 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥866 |
| 规格： | 500ug | 产品价格： | ¥5325 |
| 基因名 | E Protein |
| 别名 | E Protein |
| 蛋白号 | QHD43416.1 |
| 种属 | 2019-nCoV |
| 宿主 | E.coli |
| 产品描述 | E Protein (E.coli，N-6His) is produced by our E.coli expression system and the target gene with a 6His tag at the N-terminus. |
| 区间范围 | NA |
| 标签信息 | N-6His |
| 预测分子量 | 11 kDa |
| 表观分子量 | 11 kDa |
| 纯度 | ≥85% by SDS-PAGE. |
| 内毒素 | Not measured |
| 溶剂 | Supplied as a 0.2 μM filtered solution of 20mM Tris–HCl, 200mM NaCl, pH 8.0 |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days; -20°C for 1 months and -70℃ for 3 months. |
| 运输条件 | 干冰运输（需要收取干冰费） |
| 背景说明 | Coronavirus envelope (E) proteins are short (100 residues) polypeptides that contain at least one transmembrane (TM) domain and a cluster of 2-3 juxtamembrane cysteines.These proteins are involved in viral morphogenesis and tropism, and their absence leads in some cases to aberrant virions, or to viral attenuation.In common to other viroporins, coronavirus envelope proteins increase membrane permeability to ions, plays a central role in virus morphogenesis and assembly.Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport.Also plays a role in the induction of apoptosis.Activates the host NLRP3 inflammasome, leading to IL-1beta overproduction. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | E Protein |
| 中文名称 | E Protein蛋白 |
| 单位 | 支 |
| 规格 | 1mg ; 50ug ; 500ug |
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文献和实验Membrane Protein Insertion in E. coli
, the insertion of proteins into the membrane is catalyzed by the Sec translocase and the YidC membrane insertase. YidC can function on its own to insert proteins or together with the Sec translocase to facilitate membrane protein insertion. In this chapter
Strategies to Optimize Protein Expression in E. coli
dramatically increases the level of wild type protein expression in Escherichia coli. Protein Expr. Purif. 45:374‐380. Chang, C.N., Kuang, W.J., and Chen, E.Y. 1986. Nucleotide
Use of E. coli for the Production of a Single Protein
E. coli has been widely used for recombinant protein production. Here, we introduce a novel expression method in E. coli , the S ingle P rotein P roduction (SPP ) system, in which E. coli is converted into a bioreactor producing
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