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- 文献和实验
- 技术资料
- 抗体名:
Foxp3 Antibody 抗体
- 抗体英文名:
Foxp3 Antibody
- 靶点:
Foxp3
- 应用范围:
ELISA, IHC
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb247038
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
详询
- 免疫原:
详询
- 规格:
100 ug
描述: Goat polyclonal antibody to Foxp3
别名: anti Foxp3 antibody, anti forkhead box P3 antibody, anti MGI:1891436 antibody, anti JM2 antibody, anti scurfin antibody, anti sf antibody, anti scurfy antibody, anti RP23-54C14.1 antibody, anti OTTMUSP00000017952 antibody, anti OTTMUSP00000017961 antibody, anti OTTMUSP00000017962 antibody
应用稀释比例: ELISA: 1:16000, IHC-P: 5 μg/ml
应用说明: WB: Not yet tested. At this stage we are dependent on researchers in the field for further characterization of this product. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product.
分子量: 47.3
预测反应性: Mouse, Rat
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
蛋白序列: CTFPRSGTPRKDSN
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
研究领域: Cancer Biology
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

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文献和实验人Foxp3 ( Foxp3 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Foxp3 单抗包被于酶标板上,标准品和样品中的 Foxp3与单抗结合,加入生物素化的抗人 Foxp3 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,Foxp3 浓度与 OD 值
、ionomycin和Brefeldin A,其中前两者协同激活细胞,后者抑制蛋白转运,从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体,易非特异性结合抗体的Fc段,导致假阳性信号。为解决此问题,菲恩生物提供Purified Anti-Mouse CD16/32 Antibody(FNab30106)用于小鼠样本封闭,;Human Fc Receptor Blocking reagent(K128)用于人样本封闭。关键在于必须选择与样本
Intracellular Immunofluorescent Staining for Flow Cytometry
supernatant. [OPTIONAL] Block with 2% (2 µl) normal rat serum in 1X Permeabilization Buffer, in approximately 100 µl volume, at 4°C for 15 minutes. Without washing after blocking step, add 20 µl fluorochrome conjugated anti-human Foxp3 antibody
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