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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3年
- 英文名:
Luciferase
- 库存:
现货
- 供应商:
solarbio
- CAS号:
61970-00-1
- 规格:
1mg
品牌:Solarbio | 货号:L7840 | CAS:61970-00-1
英文名称 : Luciferase
储存条件 : -20℃
产品介绍:
recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein
Unit Definition
One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.
Physical form
Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol
recombinant expressed in E. coli
assay ≥98% (SDS-PAGE)
form buffered aqueous solution
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文献和实验of two, take the two samples transfected by your group and assay for luciferase activity. Note, in all cases, the total amount of DNA used for the transfection is 2.0µg.Luciferase assayMaterials required 1 x CCLR (cell lysis reagent) Luciferase Assay ReagentExperimental
亦称发光酶。是催化生物发光的酶系的总称。它是光物质的冷水抽提物在氧中发光时,底物虫荧光素被消耗以后残余的对热不稳定的高分子成分。现在对萤虫相海萤以及发光细菌的虫荧光素酶结晶物的研究得最多。它们属于加氧酶( oxygenase),不含金属和辅酶。对于发光,有的酶必须以 ATP等作为辅助因子,有的则不需要。其发光机制等已了解到可因种的不同而有很大的差异,虫萤光素酶具有高度的特异性,一般仅作用于来自近缘种的虫荧光素。当然,萤虫、海萤的酶是不能互相代替引起发光的。海萤的虫荧光素酶
Introduction Luciferase can be used as a reporter gene to measure the activity of promoters, and/or the transfection efficiency. Aims You will be provided with the HEK
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