- ￥4700
- GeneTex
- 美国
- GTX56252
- 2025年07月14日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the center region of CREB. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX56252
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
CREB
- 抗体英文名：
CREB antibody
- 抗体名：
CREB 抗体
- 规格：
100 μl
WB analysis of MCF7 (A), mouse brain (B), rat brain (C) whole cell lysates using GTX56252 CREB antibody.
ICC/IF analysis of formalin-fixed MCF7 cells using GTX56252 CREB antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56252 CREB antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
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文献和实验antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
of buffers prior to use. Storage Buffer for Column For 500ml 10mM Tris ph 7.5 5ml of 1M 0.3M NaCl150ml 1M or 60ml 2.5M 1mM EDTA 1ml of 0.5M 0.02% NaN3 1ml of 10% CREB Column - Purification Trial #1 Sample #5-C4-1-1 (pg.25)lysed in 4.5ml (3 vol
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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