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- Biomatik
- EKU07893
- 2025年07月12日
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文献和实验transcription activator?like effector nucleases (TALENs) are broadly useful tools for performing targeted genome editing in a wide variety of organisms and cell types including plants, zebrafish, C. elegans , rat, human somatic cells, and human pluripotent stem
to up? or downregulate expression of endogenous genes in human cells and plants. This unit describes a detailed protocol for the recently described fast ligation?based automatable solid?phase high?throughput (FLASH) assembly method. FLASH enables automated high
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
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