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- 2025年07月15日
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文献和实验All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
Brief protocol using Digest-All™ Kit is described below. The Digest-All™ is a flexible and standardized system for tissue digestion and contains trypsin, ficin, and pepsin proteolytic enzymes. The degree of digestion is based on a standardized 10 minute
Gene Knockout In Murine Embryonic Stem (ES) Cells
off, check under microscope and swirl to dislodge. Pipette the cells in trypsin with the plate at an angle to achieve a single cell suspension. Try to avoid getting bubbles. Add 4.5 ml of ES media to neutralise trypsin. Place cell suspension in a 15 ml falcon
・ Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL) ・ Immunohistochemistry (Tyner lab
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