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文献和实验Detecting Ligands Interacting with Lipoprotein Lipase
The primary role for lipoprotein lipase (LPL) is hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). This enzyme has also been implicated in the process of atherogenesis (for reviews see refs
In Vitro Transcription and Translation of Lipoprotein Lipase
LPL is regulated post-transcriptionally in response to several hormones. Post-translational regulation occurs in response to feeding (1 ,2 ). Glycosylation of LPL at the first N-linked glycosylation site is essential for catalytic activity
Techniques for the Measurement of Lipoprotein Lipase Messenger RNA
This chapter describes the measurement of lipoprotein lipase (LPL) mRNA levels in adipose tissue and muscle. Many investigators measure LPL in human biopsy samples, where only a small amount of tissue is available, requiring sensitive
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