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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询说明书
- 库存:
10
- 供应商:
上海远慕生物
- 规格:
10 x 5 ml (for 2,000 reactions of 50 µl final reaction volume)
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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
Real-Time PCR: the TaqMan Method
utilizes a system that is fairly easy to grasp conceptually. First, we must take a look at the TaqMan® probe. Figure 1. The Taqman probe. The red circle represents the quenching dye that disrupts the observable signal from the reporter dye
离上游引物的3’有一个碱基,但也可以重叠,要保证Taqman探针的5’端离上游引物的5’端至少有4bp。 如: forward primer Taqman probe 5’ → 3’ 5’ FAM→3’染料 NNNNNNN NNNNNNN 发表序列:5’-NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN-3’ 互补发表序列
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