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- 2025年12月16日
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文献和实验Targeted Sequencing Using Affymetrix CustomSeq Arrays
a basic protocol for oligo hybridization?based sequencing technology and resulting data analysis specific to the Affymetrix GeneChip CustomSeq Resequencing Array platform. All steps and critical aspects related to array design, experimental protocols, data
Cycle Sequencing Protocol Using 33P-Radiolabeled Terminators
tends to produce high background and ghost bands.) 1.Prepare the 4 termination mixes by combining 2.0ul of dGTP-termination-master-mix and 0.5ul of labeled ddNTP for each sequence. 2.For each set of sequencing reactions, label four PCR grade microfuge
RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAs
and mapping of promoters for analysis of gene expression (RAMPAGE) is a method that harnesses highly specific sequencing of 5??complete complementary DNAs to identify transcription start sites (TSSs) genome?wide. Although TSS mapping has historically relied
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