- ￥800
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- 美国
- EG-1083
- 2025年09月17日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Bst DNA Polymerase, large fragment, 8 U/µl
- 供应商：
默瑞生物
- 规格：
4000U
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文献和实验template DNA 2 μl Bst reaction buffer 2 μl Bst nucleotide extension mix 1 μl oligonucleotide primer (2.5 ng/μl) 0.5-1 μl [alpha]-32-P-dATP or [alpha]-35-S-dATP 1 μl diluted Bst polymerase (0.1 U/μl) q.s. sterile ddH2O 12 μl [alpha]-32-P
A. Bst-catalyzed radiolabeled DNA sequencingBst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by altering the absolute amounts and the relative deoxy/dideoxynucleotide ratios
Analys of Genomic DNA by Southe
) or [a -32 P]dCTP) - not provided by the kit Exo(-) Klenow fragment of DNA polymerase I (5 U/µl) Stop mix (0.5M EDTA, pH 8.0) Procedure: 10.Add to the bottom of a microfuge tube: 25-50 ng (1-23 µl) of template DNA
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