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Nitric Oxide Biosynthesis: Oxidoreductases: NOS1 (nNOS), NOS2 (iNOS), NOS3 (eNOS), NQO1.
Nitric Oxide Biosynthesis Regulation: DYNLL1, GLA, HSP90AB1 (HSPCB), IL10, INS, NOS1AP.
Other Genes: AKT1, ARG2, DDAH2, DYNLL1, EGFR, GCH1, GCHFR.
Genes Induced by Nitric Oxide: CDKN1A (p21CIP1/WAF1), IL8, JUN, VEGFA.
Genes Suppressed by Nitric Oxide: CCNA1, MYB, TROAP.
Nitric Oxide Signaling Pathway: CAMK1, DLG4 (PSD95), GRIN2D, NOS1 (nNOS), PPP3CA, PRKAR1B, PRKCA.
Superoxide Metabolism: Superoxide Release: ALOX12, DUOX1, DUOX2, NOX5, PRG3. Oxidoreductases: ALOX12, DUOX1, DUOX2, NOS2, NOX5, SOD1, SOD2, SOD3.
Peroxidases: DUOX1, DUOX2. Other Genes: CCS, NCF1, NCF2, PREX1.
Response to Oxidative Stress: Anti- Apoptosis: MPO, MTL5, NME5, PRDX2, RNF7.
Antioxidants: APOE, MT3, SELS, SOD1, SOD3, SRXN1.
Glutathione Peroxidases: GPX1, GPX2, GPX3, GPX4, GPX5, GPX6.
Oxidoreductases: CAT, EPX, GPX1, GPX2, GPX3, GPX4, GPX5, GPX6, LPO, MPO, MSRA, PRDX2, PRDX6, SOD1, SOD2, SRXN1, TPO, TXNRD2.
Peroxidases: CSDE1, CYGB, EPX, GPR156, LPO, MPO, PRDX2, PRDX5, PRDX6 (AOP2), TPO, TTN.
Transcription Regulators: CSDE1, FOXM1, GLRX2, SCRT2, SIRT2, SOD2.
Other Genes: ATOX1, DUSP1 (PTPN16), GSS, KRT1, MBL2, NUDT1, OXR1, PNKP, PRNP, SCARA3, SEPP1, SGK2.
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文献和实验PCR芯片，研究者可以同时研究大量基因，既可以进行某些特别感兴趣的信号通路的研究，也可以作为验证芯片结果的方法。 为了获得理想的实验结果，PCR芯片面临以下挑战：高特异性，高灵敏度和可重复性。高灵敏度或低检出限，在样品的总RNA量少，基因表达量低的情况下，也能检测出目的基因。高特异性，扩增产物严格保证基因特异性，避免非特异性产物，如引物二聚体的产生。可重复性，通过标准化的反应体系和实验方法，使得多人多次重复实验均能获得可靠的基因定量结果。 PCR芯片实验操作步骤 采用PCR芯片进行实验只需
Cloning and Expression of Human Inducible Nitric Oxide Synthase
Much of the excitement over nitric oxide (NO) is owing to its diverse physiologic and pathophysiologic functions. Induced NO production has been shown to have both beneficial and detrimental consequences. The inducible or high-output
., Gandley, R.E., Han, W., Wasserloos, K., Stitt, M., Kanai, A.J., McLaughlin, M.K., Pitt, B.R., and Levitan, E.S. 2000. Role of metallothionein in nitric oxide signaling as revealed by a green fluorescent fusion protein. Proc. Natl. Acad. Sci. U.S.A. 97:477
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