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文献和实验Purification of Genomic DNA Using PureLink Silica Columns
Microcentrifuge capable of centrifuging >10,000 x g PureLink™ Spin Cartridge in Collection Tubes Wash Tubes 实验材料 Lysates prepared 实验步骤 Elution Volume The DNA is eluted in 2 aliquots of 200 µl
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
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