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上海希言科学仪器有限公司
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文献和实验1) The ligation mixture contains the following: vector (~100 ng) insert (equimolar or 2 or 3 X molar concentration of vector) water added to 18 µl 2) Heat the mixture at 45 ℃ for 5 min. to melt any annealed cohesive ends. 3) Cool on ice. Add
Cohesive End Ligation 1) The ligation mixture contains the following: vector DNA (~100 ng) insert DNA (equimolar or 2 or 3 X molar concentration of vector) water added to 18 µl 2) Heat the mixture at 45 °C
1) The ligation mixture contains the following: vector DNA (~100 ng) insert DNA (equimolar or 2 or 3 X molar
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