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文献和实验Preabsorbing Antisera with C. elegans Acetone Powder
graduated cylinder, and add 5 volumes of acetone. Mix by inverting several times; should see a large amount of floculent white precipitate. Allow the precipitate to sediment (takes ~30 min), and remove the supernatant by suction. 4. Wash the precipitate
Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
increase volum by 5 ml. Increase the volume of media by 5-10 ml two times a week by aspirating off half of media from culture flask (do not to suction off cells from bottom of flask) and replacing it with fresh growth media. Cells can be harvested
Clone Genes From a Phage Library
fluid swimming on top. When putting filters on, you don't want significant air bubbles beneath them. • Write an identifier with pencil on each filter. Consider making duplicate filters for each plate to pre-verify that any signal in the end is real
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