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文献和实验A safe method of extracting DNA from Coccidioides immitis
nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1 sodium dodecyl sulfate), and incubated at 80 C for 10 min., cooled to 40 C and incubated
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
by centrifugation for 20 min at 10,000 rcf at 4℃. (Buffer S: sucrose 150 mM, KCl 40 mM, Tris/HCl 25 mM, BSA 1 mg/ml, pH 7.4) (6) Protein concentration in nuclear, cytoplasmic, and mitochondrial fractions were determined according to the Bradford method.
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