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文献和实验Spotted Microarray Hybridization and Scan
Note: for single spotted arrays halve volumes Mix two color samples (for genomic DNA, Normally 0.5ug), add 2ml Block Mix (10mg/ml salmon sperm DNA (BRL) + 4mg/ml yeast tRNA) Use Speed Vec to dry the sample
【共享】流式 Overview of Flow Cytometry
but will range from 1500 to 5000 cells/ sec. For cloning, even slower rates (∼100 to 200 cells/sec) will be required.The quality of the single-cell suspension is a significant factor in the purification and recovery of cell populations. It is important to prevent
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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