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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
7) Slowly add 10 mL of MEF-CM drop-wise to cells in the 50-mL conical tube. While adding the medium, gently move the tube back and forth to mix the hESCs. This reduces osmotic shock to the cells. 8) Rinse the vial with 1 mL of MEF-CM and add
™ working medium by adding the entire contents of the bottle of supplement to 100 mL of Base Medium. 4) The HepaRG™ working medium is now ready for use. It should be stored at 4°C for a maximum of one month. 2. Thawing and counting
Construction and Characterization of Adenovirus Vectors
medium from 150-mm dishes of 293 or A549 cells. ii. Rinse the monolayer twice with 5 mL of 1X citric saline (for 293 cells) or 2 mL of 1X trypsin-EDTA (for A549 cells). iii. For 293 cells, remove
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