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文献和实验Characterization of Potassium Channel Binding
and equipment for protein assay ( appendix 3A ) and calculation of IC 50 and K i (unit 1.3 ) Support Protocol 1: Preparation of Synaptic Plasma Membranes
384通量全自动膜片钳系统IonWorks Barracuda同时检测电压门控和配基门控离子通道
amiloride (SH),; B) Concentration-dependence of ASIC1a channel inhibition by benzamil and amiloride are determined in two different PPC experiments, with IC50 values matching literature reports. Fig 4: Recordings of ligand-gated channel currents
Spotted Microarray Hybridization and Scan
Note: for single spotted arrays halve volumes Mix two color samples (for genomic DNA, Normally 0.5ug), add 2ml Block Mix (10mg/ml salmon sperm DNA (BRL) + 4mg/ml yeast tRNA) Use Speed Vec to dry the sample
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