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文献和实验V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
, the DCC has been used to study neurite outgrowth(12). We have previously used the DCC to elucidate the role of Rho family proteinsin the chemotactic response of BAC1.2F5 macrophages to CSF-1 (13). Wedescribe the use of this chamber to study the response
of complex and protein alone with the naked eye or a light microscope, crystals of covalently Cy5?labeled DNA can be directly used for X?ray diffraction data collection. Curr. Protoc. Nucleic Acid Chem. 46:7.15.1?7.15.8. © 2011 by John Wiley & Sons
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
and Kelly (1984) and Martini et al. (1998)). These extracts are competent for in vitro DNA replication (Li and Kelly, 1984) and nucleosome assembly assays (Martini et al. 1998; Groth et al., 2005). In principal, any of your favourite lysis buffer
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