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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
cytometric cell sorting. 59. Seed 4-6 x 104 cells in 10 mL of OP9 medium per 10-cm dish of 80%-90% confluent OP9 or OP9-DL1 cells, and add 5 ng/mL Flt-3L and 1 ng/mL IL-7. Place the dishes in the incubator. The OP9 and OP9-DL1 cells
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
Plant DNA methylation analysis by bisulfite genomic sequencing
Transfer column with resin to 1.5ml reaction tube, add 50µl bidist. water preheated to 64°C for DNA elution; Incubate 1 minute at room temperature; Centrifuge Eppendorf 5414 2 minutes; To DNA solution (~50µl), add 40µl bidist. water
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