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上海希言科学仪器有限公司
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文献和实验Calibration 窗口, 根据右下角的提示进行操作。 11. 打开Manual Control, 执行 Syringe Home, 然后选择Syringe Down,输入合适的值并执行,使曲柄几乎与注射器的活塞顶部接触。 12. 具体方法:可先输入较大的值,如200,然后待靠近后,将值减少,如100, 50, 20, 5…。 三.样品准备 1. 将10 X Buffer 稀释到1 X Buffer(需约15ml)。 2. 将1 X Buffer加入
Sucrose Fractionation of Castor Bean
), attach a 2.0 ml syringe and pull out the bottom 1.5 ml of the sample. Remove the syringe and place the 1.5 ml fraction into a test tube marked as #1. Re-attach the syringe without disturbing the gradient, and repeat as often as need to totally fractionate
including the point of sample introduction. In an on-column injection, the needle must enter the column inside the oven. The injector used has a butterfly valve, which is opened as the turret of the autosampler rotates over the injector, . The sample is
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